Antiproliferative Effects of Pterodon pubescens Extract and Isolated Diterpenes in HaCaT Cells

Author(s)

Basting, Rosanna Tarkany ^{[1, 2, 3, 4]} ; de Oliveira Sousa, Ilza Maria ^{[1, 2, 3]} ; Butterweck, Veronika ^[5] ; Foglio, Mary Ann ^{[1, 3]}

Affiliation

^[1] Univ Estadual Campinas, Fac Pharmaceut Sci FCF, UNICAMP, Cidade Univ Zeferino Vaz Rua Candido Portinari 20, BR-13083871 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci FCM, Postgrad Program Med Sci, UNICAMP, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Chem Biol & Agr Res Ctr CPQBA, Paulinia, SP - Brazil

^[4] Sao Leopoldo Mand Inst & Res Ctr, Lab Neuroimmune Interface Pain Res, Campinas, SP - Brazil

^[5] Univ Appl Sci Northwestern Switzerland FHNW, Sch Life Sci, Inst Pharma Technol, Muttenz - Switzerland

• Document type: Journal article
• Source: Planta Medica; v. 87, n. 03, p. 218-224, MAR 2021.
• Web of Science Citations: 0

Abstract

Pterodon pubescens fruits are popularly used because of their analgesic and anti-inflammatory actions, which are attributed to the isolated compounds with a vouacapan skeleton. This work aimed to evaluate the antiproliferative and anti-inflammatory effects of a P. pubescens fruit dichloromethane extract and the vouacapan diterpene furan isomers mixture (1:1) (6 alpha -hydroxy-7 beta -acetoxy-vouacapan-17 beta -oate methyl ester and 6 alpha -acetoxy-7 beta -hydroxy-vouacapan-17 beta -oate methyl ester isomers) in HaCaT cells using the cell migration and the BrDU incorporation assay. Levels of IL-8 were measured by ELISA after TNF- alpha stimulation. HPLC/DAD analysis of the extract revealed the expressive presence of vouacapan diterpene furan isomers mixture. P. pubescens extract (1.5625-25 mu g/mL) and vouacapan diterpene furan isomers mixture (3.125-50 mu M) inhibited cell proliferation as indicated by a decreased BrdU-incorporation. For the evaluation of cell migration, time-lapse microscopy was used. P. pubescens presented inhibition on cell migration at all concentrations tested (3.125-12.5 mu g/mL), whereas for the VDFI mixture, the inhibition was only observed at the highest concentrations (12.5 and 25 mu M) tested. Furthermore P. pubescens extract and vouacapan diterpene furan isomers mixture significantly decreased IL-8 levels. Our results showed antiproliferative and anti-inflammatory effects on HaCaT cells treated with the extract and the vouacapan isomers mixture, without affecting cell viability. These activities could be attributed to the voucapan molecular structures. In conclusion, topical products developed of P. pubescens extract or the voucapan isomers mixture should be further studied as a potential product for local treatment against hyperproliferative lesions as in psoriasis vulgaris, representing an alternative treatment approach. (AU)

• FAPESP's process: 15/08600-2 - Pterodon pubescens Benth. and Cordia verbenacea DC: evaluation of synergestic effect in anti-inflammatory activity and studies of mechanisms of action and cell signaling
• Grantee: Rosanna Tarkany Basting
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 16/23816-4 - In vitro modulation of inflammatory target gene expression by an association of Pterodon pubescens Benth. and Cordia verbenacea DC
• Grantee: Rosanna Tarkany Basting
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)