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Evaluation of antinociceptive efficacy microencapsulated P. pubescens Benth formulation

Grant number: 11/03759-2
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2011
Effective date (End): August 31, 2013
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Mary Ann Foglio
Grantee:Rogerio Grando
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Medicinal plants have been used to treat various diseases; however, the random use can cause serious damage. Pterodon pubescens Benth. (Sucupira) seeds have been widely used by local people for treatment of various inflammatory processes. Previous studies showed that geranylgeraniol and three voucapans isolated and identified from this species were involved with anti-inflammatory and antinociceptive activity. Brazilian folkmedicine employ teas prepared from this species seeds to treat pain. The ethno pharmacological data combined with the results previously produced by our research group promoted the present study that proposed the efficacy evaluation of the aqueous P. pubescense extract on antinociceptive experimental models, stability of microparticles produced from the extract. Also since the plant species is described in folk medicine for treatment of chronic pain, arthritis experimental model induced by CFA (Complet Freund´s Adjuvants) were undertaken.Phytochemical comparison evaluation showed that the dichloromethane(Pp) extract contained geranylgeraniol, and furan diterpenes (vouacapans) whereas only vouacapans were detected in the polar aqueous extract (Ppa) . The pharmacological activity was measured by writhing induced by acetic acid, paw licking induced by formalin hyperalgesia and paw edema induced by carrageenan models. The dichloromethane extract (Pp) showed better efficacy in comparison to the aqueous extract (inhibition of writhing by 53% Ppa and 71% Pp ANOVA p <0.05; Formalin phase 1: Idle Ppa; inhibition of 77.3% Pp ANOVA p <0.05, Phase 2: Inhibition of 98.0% in Pp and 85,6 in Ppa ANOVA p <0.05. Ppa inhibited paw edema in 50% at a dose of 100mg/kg Pp and Ppa. ANOVA p <0.05) therefore the nonpolar extract (Pp) was chosen to go forward with the arthritis experimental model. Furthermore this data corroborates previous studies that suggest a synergistic effect of geranylgeraniol and vouacapans on the antinociceptive activity . The dichlorometanic extract (Pp) was tested using 30, 100 and 300 mg/kg oral doses during 14 days treatment . The results indicated that Pp protected rats against CFA induced arthritis by decreasing paw volume (30% compared to control group ANOVA p>0,05) and increasing nociceptive threshold (42,84% compared to control group ANOVA p>0,005). The extract showed no toxic effect against liver and kidneys demonstrating immunosuppressive effect by reducing significant dose-dependent lymphocytes. The present investigation, suggest that Pp extract has an anti-arthritic activity. Moreover sub-acute treatment did not cause significant changes in biochemical parameters. However further studies are still required to assess the possible immunosuppressive action of this extract (AU)

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.

Filed patent(s) as a result of this research project

COMPOSIÇÃO À BASE DE PTERODON PUBESCENS BENTH., SUA FORMULAÇÃO E USOS BR1020130210960 - Universidade Estadual de Campinas (UNICAMP) . Leila Servat; Ilza Maria Oliveira Souza; Mary Ann Foglio; Rogério Grando; Humberto Moreira Spindola; Nubia de Cassia Almeida Queiroz; Vanessa Helena da Silva Souza; Karin Maia Monteiro; Rodney Alexandre Ferreira Rodrigues - August 2013, 19

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1320180763783 - Universidade Estadual de Campinas (UNICAMP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - December 2018, 18