Synergy of Biodegradable Polymer Coatings with Quaternary Ammonium Salts Mediating Barrier Function Against Bacterial Contamination and Dehydration of Eggs

Author(s):Show less -

Almeida E Silva, Taiane ^[1] ; Gorup, Luiz Fernando ^{[2, 3, 4]} ; de Araujo, Renata Pires ^{[3, 5]} ; Fonseca, Gustavo Graciano ^[5] ; Martelli, Silvia Maria ^[1] ; Pires de Oliveira, Kelly Mari ^{[3, 5]} ; Faraoni, Lucas Henrique ^[1] ; Ratier de Arruda, Eduardo Guimaraes ^[6] ; Borges Gomes, Raphael Antonio ^[7] ; Manoel da Silva, Carlos Henrique ^[3] ; de Arruda, Eduardo Jose ^{[1, 2, 3, 5]}

Affiliation

^[1] Fed Univ Grande Dourados, Fac Engn FAEN, BR-79804970 Dourados, MS - Brazil

^[2] Univ Fed Sao Carlos, LIEC Dept Chem, Rod Washington Luis Km 235, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[3] Fed Univ Grande Dourados, Fac Exact Sci & Technol FACET, BR-79804970 Dourados, MS - Brazil

^[4] Univ Fed Pelotas, Mat Engn, Campus Porto, BR-96010610 Pelotas, RS - Brazil

^[5] Fed Univ Grande Dourados, Fac Biol & Environm Sci FCBA, BR-79804970 Dourados, MS - Brazil

^[6] Univ Fed Sao Carlos, Dept Med, Rod Washington Luis Km 235, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[7] Univ Fed Ouro Preto, Sch Nutr, Rua Dois, Campus Morro Cruzeiro, BR-35400000 Ouro Preto, MG - Brazil

• Document type: Journal article
• Source: Food and Bioprocess Technology; v. 13, n. 12, p. 2065-2081, DEC 2020.
• Web of Science Citations: 0

Abstract

Eggs are an important, low-cost, high-protein food with a balanced nutritional composition. The sanitary quality of commercial eggs is a bottleneck that needs to be overcome through the use of technologies that guarantee the safety of the product. This work aimed to prepare, characterize, and evaluate the coating of commercial eggs by chitosan filmogenic solutions and 1st- and 5th-generation quaternary ammonium salt components (QACs). Egg shell properties such as thickness, morphology, and wettability were analyzed by scanning electron microscopy (SEM),TG/DSC, pH(PZC), contact angle, color, and weight loss. Determinations of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) with Salmonella spp., Escherichia coli, and Staphylococcus aureus were performed, as well as proliferation tests on eggshell surfaces. The results show a synergistic effect of the biopolymer in terms of antibacterial activity against all microorganisms tested. The composite of chitosan and ammonium quaternary ammonium films offers physical and biological safety to guarantee the integrity and microbiological quality of commercial eggs. Egg coating using bioactive chitosan films was shown to be a suitable method of preservation and a physical and microbiological barrier to minimize egg quality loss during storage, as well as to increase shelf life. (AU)

• FAPESP's process: 08/57872-1 - National Institute for Materials Science in Nanotechnology
• Grantee: Elson Longo da Silva
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/23572-0 - Nanostructured hybrid materials by self-assembly of nanoparticles induced by biomolecules
• Grantee: Luiz Fernando Gorup
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/07067-0 - Photocatalyst based in bismuth titanate synthesized by the oxidant peroxo method (OPM)
• Grantee: Emerson Rodrigues de Camargo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
• Grantee: Elson Longo da Silva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC