The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish,Astyanax altiparanae(Characiformes, Characidae)

Author(s)

Postingel Quirino, Patricia ^{[1, 2]} ; da Silva Rodrigues, Maira ^[1] ; da Silva Cabral, Elis Marina ^[1] ; de Siqueira-Silva, Diogenes Henrique ^[3] ; Mori, Ricardo Hideo ^[1] ; Butzge, Arno Juliano ^[4] ; Nobrega, Rafael Henrique ^[4] ; Ninhaus-Silveira, Alexandre ^[1] ; Verissimo-Silveira, Rosicleire ^[1]

Affiliation

^[1] Univ Estadual Paulista, UNESP, LINEO Lab Neotrop Ichthyol, Dept Biol & Zootecnia, Campus Ilha Solteira, Rua Moncao 226, BR-15385000 Ilha Solteira, SP - Brazil

^[2] Inst Biociencias Botucatu UNESP IBB, Programa Posgrad Ciencias Biol Zool, R Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

^[3] UNIFESSPA Univ Fed Sul & Sudeste Para, Res Grp Reprod Amazon Fish GERPA LaNec, Inst Estudo Saude Biol IESB, Folha 31, Quadra 07, Lote Especial S-N, BR-68507590 Maraba - Brazil

^[4] UNESP Univ Estadual Paulista Julio Mesquita de Fi, Reprod & Mol Biol Grp, Dept Morfol, Inst Biociencias Botucatu, Campus Botucatu, BR-18618689 Botucatu, SP - Brazil

• Document type: Journal article
• Source: FISH PHYSIOLOGY AND BIOCHEMISTRY; v. 47, n. 3, p. 747-755, JUN 2021.
• Web of Science Citations: 2

Abstract

In view of the established climate change scenario and the consequent changes in global temperature, it is essential to study its effects on animal spermatogenesis. Therefore, the aim of this study was to verify the duration of spermatogenesis at different temperatures. For this purpose, 96 male and adult specimens ofAstyanax altiparanaewere kept in a closed circulation system with water temperature stabilized at 27 degrees C and 32 degrees C. Subsequently, the specimens received pulses of BrdU (bromodeoxyuridine) at a concentration of 100 mg/kg/day for 2 consecutive days, and the samples were collected daily for a period of 15 days. Their testes were removed, fixed, processed in historesin, and sectioned in 3 mu m, submitted to hematoxylin/eosin staining and to bromodeoxyuridine immunodetection. Partial results of the optimum temperature experiments allowed the classification ofA. altiparanaespermatogenic cells in A(und), A(diff), and type B spermatogonia, spermatocytes, spermatids, and spermatozoa. The duration of spermatogenesis was determined as approximately 6 days for animals at a temperature of 27 degrees C and 1 day for animals at 32 degrees C. The elevated temperature was also responsible for increasing cell proliferation, resulting in an increase in the number of spermatocytes, spermatids, spermatozoa, and cell death (cell pyknotic). The duration of spermatogenesis inA. altiparanaewas directly affected by the elevated water temperature, causing a reduction in the estimated time of spermatogenesis. (AU)

• FAPESP's process: 14/23379-8 - Xenogenic transplantation of spermatogonial stem cells in Characidae (Teleostei, Characiformes)
• Grantee: Rosicleire Veríssimo Silveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/24527-8 - Histological and stereological evaluation of Astyanax altiparanae (Characiformes, Characidae) spermatogenesis
• Grantee: Maira da Silva Rodrigues
• Support Opportunities: Scholarships in Brazil - Scientific Initiation