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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model

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Author(s):
Sarmiento-Machado, Luis Manuel [1] ; Romualdo, Guilherme Ribeiro [2] ; Zapaterini, Joyce Regina [1] ; Tablas, Mariana Baptista [1] ; Henrique Fernandes, Ana Angelica [3] ; Moreno, Fernando Salvador [4] ; Barbisan, Luis Fernando [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Biosci Inst, Dept Morphol, UNESP, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Med Sch, Dept Pathol, UNESP, Botucatu, SP - Brazil
[3] Sao Paulo State Univ, Biosci Inst, Dept Chem & Biochem, UNESP, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL; v. 73, n. 5, p. 817-828, MAR 10 2021.
Web of Science Citations: 1
Abstract

Capsaicin (CPS), an ingredient of Capsicum plants, has anti-inflammatory, antioxidant and antitumoral properties. The mechanisms of CPS on hepatocarcinogenesis preclinical bioassays are not described. Thus, the protective effects CPS were evaluated in the early stages of chemically-induced hepatocarcinogenesis. Male Wistar rats received diet containing 0.01% or 0.02% CPS for 3 weeks. Afterwards, animals received a dose of hepatocarcinogen diethylnitrosamine (DEN, 100 mg/kg body weight). From weeks 4-12, groups had their diet replaced by a 0.05% phenobarbital supplemented one to promote DEN-induced preneoplastic lesions. Animals were euthanized 24 h after DEN administration (n = 5/group) or at week 12 (n = 9/group). The estimated CPS intake in rats resembled human consumption. At the end of week 3, dietary 0.02% CPS attenuated DEN-induced oxidative damage and, consequently, hepatocyte necrosis by reducing serum alanine aminotransferase levels, liver CD68-positive macrophages, lipid peroxidation, while increasing antioxidant glutathione system. Additionally, 0.02% CPS upregulated vanilloid Trpv1 receptor and anti-inflammatory epoxygenase Cyp2j4 genes in the liver. Ultimately, previous 0.02% CPS intake decreased the number of GST-P-positive preneoplastic lesions at week 12. Thus, CPS attenuated preneoplastic lesion development, primarily by diminishing DEN-induced oxidative liver injury. Findings indicate that CPS is a promising chemopreventive agent when administered after and during the early stages of hepatocarcinogenesis. (AU)

FAPESP's process: 18/19432-1 - Molecular and functional analysis of mammary carcinogenesis related to maternal protein restriction
Grantee:Joyce Regina Zapaterini Rossi
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/12015-0 - Caffeine, Trigonelline and Chlorogenic Acid: Modulation of miRNA expression in Fibrosis-associated Hepatocarcinogenesis.
Grantee:Guilherme Ribeiro Romualdo
Support Opportunities: Scholarships in Brazil - Doctorate