Effects of lithium and selenium in the tail muscle of American bullfrog tadpoles (Lithobates catesbeianus) during premetamorphosis

Author(s)

Pinto-Vidal, Felipe Augusto ^{[1, 2, 3]} ; Carvalho, Cleoni dos Santos ^{[2, 4]} ; Abdalla, Fabio Camargo ^{[2, 5]} ; Moraes Utsunomiya, Heidi Samantha ^[4] ; Salla, Raquel Fernanda ^[6] ; Jones-Costa, Monica ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Carlos, Dept Biol, Lab Physiol Conservat LaFisC, Sorocaba - Brazil

^[2] Univ Fed Sao Carlos, Postgrad Program Biotechnol & Environm Monitoring, Sorocaba - Brazil

^[3] Masaryk Univ, Fac Sci, RECETOX, Kamenice 753-5, Pavil D29, Brno 62500 - Czech Republic

^[4] Univ Fed Sao Carlos, Lab Biochem & Microbiol LaBioM, Sorocaba - Brazil

^[5] Univ Fed Sao Carlos, Lab Struct & Funct Biol LaBEF, Sorocaba - Brazil

^[6] Univ Estadual Campinas, Lab Nat Hist Brazilian Amphibians LaHNAB, Campinas - Brazil

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. 29, n. 2 AUG 2021.
• Web of Science Citations: 0

Abstract

The amphibian populations have faced a drastic decline over the past decades. This decline has been associated with the presence of contaminants in the environment, among other environmental stressors. The present study tested the responses following the exposure to lithium (2.5 mg L-1) and selenium (10 mu g L-1), both isolated and as a mixture, on the metabolic status of the tail muscle of premetamorphic American bullfrog (Lithobates catesbeianus) through the assessment of the total protein content, mobilization of glucose and triglycerides, and the activity of lactate dehydrogenase (LDH). The exposure followed a 21-day assay with two sampling periods (on the 7th and 21st day after the onset of exposure) to evaluate the effects over time. The group exposed to the mixture presented a statistically decreased LDH activity (P < 0.05) in both sampling periods. The presence of selenium elicited a statistically significant increase (P < 0.05) in the glucose mobilization after 7 days of exposure. After 21 days, the animals exposed to selenium presented levels of glucose mobilization comparable to the control group. The mobilization of glucose and triglycerides remained similar to the control group for the animals exposed to lithium and to the mixture in both periods of sampling (P > 0.05). The total protein content did not show any statistical difference in the treated groups throughout the experiment (P > 0.05). The presented results highlight the importance of the assessment of mixtures that can occur in the environment, since the combination of contaminants may elicit distinct toxicity compared with the effects triggered by the chemicals isolated. (AU)

• FAPESP's process: 08/57712-4 - The National Institute of Comparative Physiological Research
• Grantee: Augusto Shinya Abe
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/03653-6 - Sixth International Conference on Environmental Management, Engineering, Planning and Economics (CEMEPE) and to the SECOTOX
• Grantee: Fábio Camargo Abdalla
• Support Opportunities: Research Grants - Meeting - Abroad