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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

LOXL3 Silencing Affected Cell Adhesion and Invasion in U87MG Glioma Cells

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Laurentino, Talita de S. [1] ; Soares, Roseli da S. [1] ; Lerario, Antonio M. [2] ; Marie, Suely K. N. [1] ; Oba-Shinjo, Sueli M. [1]
Total Authors: 5
[1] Univ Sao Paulo, Fac Med FMUSP, Neurol Dept, Cellular & Mol Biol Lab LIM 15, BR-01246000 Sao Paulo, SP - Brazil
[2] Univ Michigan, Dept Internal Med, Div Metab Endocrinol & Diabet, Ann Arbor, MI 48109 - USA
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

Lysyl oxidase-like 3 (LOXL3), belonging to the lysyl oxidase family, is responsible for the crosslinking in collagen or elastin. The cellular localization of LOXL3 is in the extracellular space by reason of its canonical function. In tumors, the presence of LOXL3 has been associated with genomic stability, cell proliferation, and metastasis. In silico analysis has shown that glioblastoma was among tumors with the highest LOXL3 expression levels. LOXL3 silencing of U87MG cells by siRNA led to the spreading of the tumor cell surface, and the transcriptome analysis of these cells revealed an upregulation of genes coding for extracellular matrix, cell adhesion, and cytoskeleton components, convergent to an increase in cell adhesion and a decrease in cell invasion observed in functional assays. Significant correlations of LOXL3 expression with genes coding for tubulins were observed in the mesenchymal subtype in the TCGA RNA-seq dataset of glioblastoma (GBM). Conversely, genes involved in endocytosis and lysosome formation, along with MAPK-binding proteins related to focal adhesion turnover, were downregulated, which may corroborate the observed decrease in cell viability and increase in the rate of cell death. Invasiveness is a major determinant of the recurrence and poor outcome of GBM patients, and downregulation of LOXL3 may contribute to halting the tumor cell invasion. (AU)

FAPESP's process: 16/05777-1 - Functional role of enzyme LOXL3 in astrocytoma
Grantee:Talita de Sousa Laurentino
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/03614-5 - Functional detailing of CD99 role in astrocytomas
Grantee:Sueli Mieko Oba Shinjo
Support type: Regular Research Grants