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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MagC is a NplC/P60-like member of the alpha-2-macroglobulin Mag complex of Pseudomonas aeruginosa that interacts with peptidoglycan

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Author(s):
Zouhir, Samira [1] ; Contreras-Martel, Carlos [2] ; Maragno Trindade, Daniel [1] ; Attree, Ina [3] ; Dessen, Andrea [1, 2] ; Macheboeuf, Pauline [2]
Total Authors: 6
Affiliation:
[1] CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas - Brazil
[2] Univ Grenoble Alpes, CNRS, CEA, IBS, Grenoble - France
[3] Univ Grenoble Alpes, CNRS, INSERM, Unite Biol Cellulaire & Infect, CEA, Grenoble - France
Total Affiliations: 3
Document type: Journal article
Source: FEBS Letters; v. 595, n. 15, p. 2034-2046, AUG 2021.
Web of Science Citations: 0
Abstract

Bacterial alpha-2 macroglobulins (A2Ms) structurally resemble the large spectrum protease inhibitors of the eukaryotic immune system. In Pseudomonas aeruginosa, MagD acts as an A2M and is expressed within a six-gene operon encoding the MagA-F proteins. In this work, we employ isothermal calorimetry (ITC), analytical ultracentrifugation (AUC), and X-ray crystallography to investigate the function of MagC and show that MagC associates with the macroglobulin complex and with the peptidoglycan (PG). However, the catalytic residues of MagC display an inactive conformation that could suggest that it binds to PG but does not degrade it. We hypothesize that MagC could serve as an anchor between the MagD macroglobulin and the PG and could provide stabilization and/or regulation for the entire complex. (AU)

FAPESP's process: 13/01962-0 - Structure and function of bacterial virulence factors
Grantee:Samira Zouhir
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/12436-9 - ANTIBIO-BAC: exploring the bacterial cell wall as a target for novel antibiotherapies
Grantee:Andrea Dessen de Souza e Silva
Support Opportunities: Research Projects - SPEC Program
FAPESP's process: 11/52067-6 - Assembly and structure of macromolecular complexes involved in bacterial cell wall: biosynthesis and virulence
Grantee:Andrea Dessen de Souza e Silva
Support Opportunities: Research Projects - SPEC Program