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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species

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Author(s):
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Salerno, Tatiana [1] ; Siqueira, Amanda Keller [1] ; de Almeida Nogueira Pinto, Jose Paes [1] ; Ribeiro de Souza da Cunha, Maria de Lourdes [2] ; Silvestre, Patricia Kelly [3] ; Zeni Condas, Larissa Anuska [1] ; Batista Lara, Gustavo Henrique [1] ; Pereira, Juliano Goncalves [1] ; da Silva, Aristeu Vieira [4] ; Paganini Listoni, Fernando Jose [1] ; Araujo Martins, Lorrayne de Souza [1] ; Motta, Rodrigo Garcia [1] ; Ribeiro, Marcio Garcia [1]
Total Authors: 13
Affiliation:
[1] Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Prod Anim & Med Vet Prevent, Botucatu, SP - Brazil
[2] Univ Estadual Paulista, Inst Biociencias, Dept Microbiol, Botucatu, SP - Brazil
[3] Univ Estadual Paulista, Fac Med, Botucatu, SP - Brazil
[4] Univ Feira Santana, Dept Ciencias Biol, Feira De Santana, BA - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 63, 2021.
Web of Science Citations: 0
Abstract

ABSTRACT Many infants are nurtured with milk supplied by human banks, whose bacteriological and physical-chemical profiles are a major issue. We investigated the bacteriological and physical-chemical characteristics, as well as genotypic and phenotypic and profiles of Staphylococcus species isolated from 240 samples of breast milk from a bank in a teaching hospital. Dornic acidity of milk revealed that 95.4% (229/240) had acceptable limits (< 8.0 oD). Caloric intake showed a wide variation in cream content (4%), fat (4%) and energy values (559.81 Kcal/L). Staphylococcus (105/186 or 56.5%) and Enterobacter (25/186 or 13.4%) were the most prevalent genera, although other microorganisms were identified, including Klebsiella pneumoniae and Pseudomonas aeruginosa. Amoxicillin/clavulanic acid (125/157 or 79.6%), vancomycin (115/157 or 73.2%), and cephalexin (112/157 or 71.3%) were the most effective antimicrobials. High resistance rates of isolates were found to penicillin G (141/157 or 89.8%), ampicillin (135/157 or 86%), and oxacillin (118/157 or 75.2%). Multidrug resistance to ≥ 3 antimicrobials occurred in 66.2% (123/186) of the isolates. Residues of microbial multiplication inhibitory substances were found in 85% (204/240) of samples. Among the coagulase-positive-CPS and negative-CoNS staphylococci, the mecA gene was detected in 53.3% (8/15) and 75% (30/40), respectively. Genes sea, seb and sec were detected in 20% (3/15) of CPS, while tsst-1 was detected in 13.34% (2/15). In addition, 13.3% (2/15) of S. aureus were toxin-producers. Genes sea, seb and sec were detected in 90% (36/40), 5% (2/40) and 15% (6/40) CoNS, respectively. Enterotoxin production was identified in 5% (2/40) of CoNS. The identification of multidrug-resistant bacteria, staphylococci species toxin-producers harboring methicillin-resistance genes, and residues of microbial multiplication inhibitory substances reinforce the need for a continuous vigilance of milk quality offered to infant consumption by human banks. (AU)

FAPESP's process: 10/50823-5 - Microbiological evaluation, physical-chemistries and antimicrobials residues detention in human, bovine and goat milk, and research virulence factors in Staphylococcus spp and Escherichia coli strains
Grantee:Marcio Garcia Ribeiro
Support Opportunities: Regular Research Grants