Molecular epidemiology of oxacillin-resistant Staphylococcus aureus and coagulase negative staphylococci from bulk tank milk from dairy herds

• Grant number: 11/09106-0
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2012 - August 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Maria de Lourdes Ribeiro de Souza da Cunha
• Grantee: Maria de Lourdes Ribeiro de Souza da Cunha
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Danilo Flávio Moraes Riboli ; Patrícia Yoshida Faccioli Martins

Abstract

Milk and dairy products are very important in human nutrition. It is necessary to obtain these products with microbiological quality and safety to health. Mastitis is one constant problem in dairy production, that affects this quality, and can be caused by several pathogens. We can highlight the genus Staphylococcus which presents many virulence factors. Thus, the aim of this work is to study the isolates from 33 bulk tank milk from dairy farms localized in cities next to Botucatu - SP and 1 bulk tank milk in Nova Odessa city - SP, to identify correctly the Staphylococcus spp species through phenotypic and genotypic methods, comparing with the staphylococci identification results obtained with a quick identification system; to verify the presence of the encoding genes to PVL toxin, the presence of the encoding gene mecA to resistance to methicillin and its SCCmec; to determine the antimicrobial resistance, including oxacillin, by E-test; to typify the methicillin-resistant isolates by PFGE, analyzing the similarity and presence of clones between the farms. Therefore, it will be possible to evaluate the dairy herd importance for the transmission of staphylococci with important characteristics of resistance and pathogenicity to man, as the MRSA, in addition to compare the clones existent in different farms with themselves and with the other clones found in different studies. (AU)