Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients

Author(s):Show less -

Saad Menezes, Maria Clara ^[1] ; Muller Veiga, Alicia Dudy ^[1] ; de Lima, Thais Martins ^[1] ; Kubo Ariga, Suely Kunimi ^[1] ; Barbeiro, Hermes Vieira ^[1] ; Moreira, Claudia de Lucena ^[1] ; Sardinha Pinto, Agnes Araujo ^[1] ; Brandao, Rodrigo Antonio ^[1] ; Marchini, Julio Flavio ^[1] ; Alencar, Julio Cesar ^[1] ; Marino, Lucas Oliveira ^[1] ; Gomez, Luz Marina ^[1] ; Saraiva Camara, Niels Olsen ^[2] ; Souza, Heraldo P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med, Emergency Med Dept, Rua Dr Arnaldo 455, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 11, n. 1 JUL 27 2021.
• Web of Science Citations: 0

Abstract

The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n=50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n=29); a control group (CONTROL, n=17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-alpha), interferon beta (IFN-beta), interferon gamma (IFN-gamma), interferon lambda (IFN-lambda), pro-interleukin(IL)-1 beta (pro-IL-1 beta), and IL-18 was determined on admission, between 5-9 days, and between 10-15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4. (AU)

• FAPESP's process: 20/04738-8 - Patients with Severe Acute Respiratory Syndrome due to COVID-19 in an emergency department
• Grantee: Heraldo Possolo de Souza
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/14566-4 - Diagnostic and prognostic markers in patients admitted to an emergency department
• Grantee: Heraldo Possolo de Souza
• Support Opportunities: Research Projects - Thematic Grants