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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photobiomodulation therapy does not depend on the differentiation of dental pulp cells to enhance functional activity associated with angiogenesis and mineralization

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Author(s):
Chimello-Sousa, Daniela Thomazatti [1] ; Lavez, Geovane Praxedes [1] ; Fernandes, Roger Rodrigo [2] ; Tavares, Milla Sprone [2] ; Rosa, Adalberto Luiz [2] ; Siessere, Selma [1] ; Hallak Regalo, Simone Cecilio [1] ; Bombonato-Prado, Karina Fittipaldi [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol, Bone Res Lab, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Lasers in Medical Science; v. 36, n. 9 AUG 2021.
Web of Science Citations: 0
Abstract

The purpose of this study is to analyze the influence of InGaAlP diode laser (660 nm) with or without an odontogenic medium (OM) in the functional activity of OD-21 cells. Undifferentiated OD-21 pulp cells were cultivated with or without OM and divided into four groups (n = 5): nonirradiated control (C -), nonirradiated + OM (C +), irradiated (L -), and irradiated + OM (L +). Laser application was performed in two sessions of a 24-h interval with an irradiance of 11.3 mW/cm2, energy density of 1 J/cm(2), and total cumulative energy/well of 4.6 J. Cell proliferation, VEGF-164 expression, mineralization, and expression of Alp, Runx2, and Dmp1 genes, as well as immunolocalization of RUNX2 and MEPE proteins, were evaluated. Data were analyzed by statistical tests (alpha = 0.05). All studied groups showed a similar increase in cell proliferation with or without OM. After 7 and 10 days, a significatively higher concentration of VEGF-164 in L - group when compared to C - group was observed. A significant increase in mineralized nodules in the L + was noted when compared to C + in the same conditions. Photobiomodulation upregulated significantly Runx2 and Dmp1 expression after 10 days in L - and after 7 days in L + , with downregulation of Dmp1 after 10 days in L + group. Immunolocalization of RUNX2 and MEPE was expressive after 7 days of culture in the cytoplasm adjacent to the nucleus with a decrease after 10 days, regardless of the presence of OM. Photobiomodulation enhances metabolism associated with angiogenesis, gene expression, and mineralization regardless of the odontogenic medium in OD-21 cells. (AU)

FAPESP's process: 10/50086-0 - In vitro evaluation of dental pulp stem cells (OD-21) after irradiation with gallium aluminum arsenide laser (GaAlAs)
Grantee:Simone Cecilio Hallak Regalo
Support type: Regular Research Grants