Effect of a sugarcane cystatin on the profile and viability of microcosm biofilm and on dentin demineralization

Author(s):Show less -

Frazao Camara, Joao Victor ^[1] ; Araujo, Tamara Teodoro ^[1] ; Mendez, Daniela Alejandra Cusicanqui ^[1] ; da Silva, Natara Dias Gomes ^[1] ; de Medeiros, Felipe Favaro ^[1] ; Santos, Lethycia Almeida ^[1] ; de Souza Carvalho, Thamyris ^[1] ; Reis, Fabiana Navas ^[1] ; Martini, Tatiana ^[1] ; Moraes, Samanta Mascarenhas ^[1] ; Shibao, Priscila Yumi Tanaka ^[2] ; Groisman, Sonia ^[3] ; Magalhaes, Ana Carolina ^[1] ; Henrique-Silva, Flavio ^[2] ; Buzalaf, Marilia Afonso Rabelo ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Dr Octavio Pinheiro Brisolla, BR-17011220 Bauru, SP - Brazil

^[2] Univ Fed Sao Carlos, Rod Washington Luiz S-N, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Rio de Janeiro, Sch Dent, Cidade Univ, R Prof Rodolpho Paulo Rocco 325, BR-21941617 Rio De Janeiro, RJ - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 203, n. 7, p. 4133-4139, SEP 2021.
• Web of Science Citations: 1

Abstract

To analyze the effect of a sugarcane cystatin (CaneCPI-5) on the microbial profile and viability, as well as on the prevention of dentin demineralization using a microcosm biofilm model. Ninety bovine dentine specimens were divided into five experimental groups according with the solution they were treated for 60 s: (1) PBS (negative control), (2) 0.12% chlorhexidine (positive control), (3) Fluoride (500 ppm F, as NaF), (4) 0.025 mg/ml CaneCPI-5, and (5) 0.05 mg/ml CaneCPI-5. Specimens were incubated with inoculum (McBain's saliva plus human saliva) in the first 8 h, and from then on, they were exposed to McBain saliva containing sucrose and daily treated (60 s) with the solutions for 5 days. Resazurin and colony-forming unit counting assays were performed. Dentin demineralization was measured by transverse micro-radiography (TMR). 0.12% chlorhexidine significantly reduced the metabolic activity of the microcosm biofilm in relation to the negative control and treated groups (p < 0.01). CHX and F significantly reduced the counts of total microorganisms, mutans group streptococci, and lactobacilli when compared with the negative control. None of the treatments was able to significantly reduce dentin demineralization in comparison with the negative control. In the model evaluated, CaneCPI-5 neither altered the microcosm biofilm profile and viability nor protected dentin against demineralization. (AU)

• FAPESP's process: 19/16247-1 - Evaluation of effect of a new cystatin derived from sugarcane on the profile and viability of microcosm biofilm, as well and on dentin demineralization preventio
• Grantee: Felipe Fávaro de Medeiros
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/24450-9 - Evaluation of effect of a new cystatin derived from sugar cane on the profile and viability of microcosm biofilm, as well and on dentin demineralization prevention
• Grantee: João Victor Frazão Câmara
• Support Opportunities: Scholarships in Brazil - Master