Molecular detection of Apicomplexan hemoparasites in anurans from Brazil

Author(s)

Ferreira, Daniel de Abreu Reis ^[1] ; Perles, Livia ^[2] ; Machado, Rosangela Zacarias ^[2] ; Prado, Cynthia P. A. ^[1] ; Andre, Marcos Rogerio ^[2]

Affiliation

^[1] UNESP, Fac Agr & Vet Sci, FCAV, Dept Morphol & Anim Physiol, Jaboticabal, SP - Brazil

^[2] UNESP, Fac Ciencias Agr & Vet Julio de Mesquita Filho, Dept Patol Vet, Lab Imunoparasitol, Campus Jaboticabal, BR-14884900 Jaboticabal, SP - Brazil

• Document type: Journal article
• Source: Parasitology Research; v. 119, n. 10, p. 3469-3479, OCT 2020.
• Web of Science Citations: 2

Abstract

Amphibians are among the most threatened vertebrate groups in the world, and the main causes include climate change, habitat destruction, and emerging diseases. Herein, we investigated the occurrence and characterized molecularly Apicomplexa in anurans from southeastern Brazil. Forty individuals from seven anuran species were sampled in Sao Paulo state. In the molecular analyses, oneLeptodactylus latransand oneRhinella diptychawere positive in PCR assays for species ofHepatozoon. TwoL. latranswere also positive for coccidian infections (Lankesterellasp.and an unidentified coccidian species). Phylogenetic analysis based on 18S rDNA clustered the sequences detected in anurans from the present study withHepatozoonspp. detected in reptiles and other anurans from Brazil, albeit they were separate fromHepatozoonhaplotypes detected in frogs from Africa and North America. Our study showed, for the first time, the molecular detection ofLankesterellasp. and another coccidian inL. latrans. Additionally, co-infection by different species ofHepatozoonhaplotypes and an unidentified coccidian in anurans from Brazil was documented. (AU)

• FAPESP's process: 18/02753-0 - ISOLATION AND GENOTYPING OF Bartonella spp. IN DOMESTIC AND WILD RESERVOIR MAMMALS IN BRAZIL
• Grantee: Marcos Rogério André
• Support Opportunities: Regular Research Grants