| Full text | |
| Author(s): Show less - |
Santos, Kamila R.
[1]
;
Souza, Fernando N.
[2, 1]
;
Ramos-Sanchez, Eduardo M.
[3, 4]
;
Batista, Camila F.
[1]
;
Reis, Luiza C.
[3]
;
Fotoran, Wesley F.
[5]
;
Heinemann, Marcos B.
[6]
;
Goto, Hiro
[7, 3]
;
Gidlund, Magnus
[8]
;
Cunha, Adriano F.
[9]
;
Faria, Angelica Rosa
[10, 11]
;
Andrade, Helida M.
[10]
;
Lage, Andrey P.
[12]
;
Cerqueira, Monica M. O. P.
[9]
;
Della Libera, Alice M. M. P.
[1]
Total Authors: 15
|
| Affiliation: Show less - | [1] Univ Sao Paulo, Dept Clin Med, Vet Clin Immunol Res Grp, Fac Med Vet & Zootecnia, BR-05508270 Sao Paulo - Brazil
[2] Univ Fed Paraiba, Programa Posgrad Ciencia Anim, BR-58397000 Areia - Brazil
[3] Univ Sao Paulo, Lab Soroepidemiol & Imunobiol, Inst Med Trop, Fac Med, BR-05403000 Sao Paulo - Brazil
[4] Univ Nacl Toribio Rodriguez Mendoza Amazonas, Dept Salud Publ, Fac Ciencias Salud, Chachapoyas 01000 - Peru
[5] Inst Butantan, Lab Genet, Div Biol, BR-05503900 Sao Paulo - Brazil
[6] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, BR-05508270 Sao Paulo - Brazil
[7] Univ Sao Paulo, Dept Med Prevent, Fac Med, BR-01246903 Sao Paulo - Brazil
[8] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508900 Sao Paulo - Brazil
[9] Univ Fed Minas Gerais, Dept Tecnol & Inspecao Prod Origem Anim, Escola Vet, BR-31270010 Belo Horizonte, MG - Brazil
[10] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG - Brazil
[11] Univ Fed Alfenas, Lab Parasitol Clin, Fac Ciencias Farmaceut, BR-37130000 Alfenas, MG - Brazil
[12] Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, BR-31270010 Belo Horizonte, MG - Brazil
Total Affiliations: 12
|
| Document type: | Journal article |
| Source: | VACCINES; v. 9, n. 8 AUG 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit alpha (SAS), succinyldiaminopimelate (SDD), and cysteinyl-tRNA synthetase (CTS) recombinant proteins; and G4: SAS+SDD+CTS plus GM-CSF DNA vaccine. The lymphocyte subpopulations, and the intracellular interleukin-17A (IL-17A) and interferon-gamma production in the draining lymph node cells were immunophenotyped by flow cytometry. The immunophenotyping and lymphocyte proliferation was determined in spleen cells cultured with and without S. aureus stimulus. Immunization with S. aureus recombinant proteins generated memory cells in draining lymph nodes. Immunization with the three recombinant proteins plus GM-CSF DNA led to an increase in the percentage of IL-17A(+) cells among overall CD44(+) (memory), T CD4(+), CD4(+) T CD44(+) CD27(-), gamma delta TCR, gamma delta TCR+ CD44(+) CD27(+), and TCRV gamma 4(+) cells. Vaccination with S. aureus recombinant proteins associated with GM-CSF DNA vaccine downregulated T(H)2 immunity. Immunization with the three recombinant proteins plus the GM-CSF DNA led to a proliferation of overall memory T, CD4(+), and CD4(+) TEM cells upon S. aureus stimulus. This approach fostered type 3 immunity, suggesting the development of a protective immune response against S. aureus. (AU) | |
| FAPESP's process: | 18/23572-3 - Evaluation of the immunogenicity of recombinant proteins predicting cure and prevention of new intramammary infections by Staphylococcus aureus in dairy cows |
| Grantee: | Camila Freitas Batista |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/23189-4 - Intramammary infections by Staphylococcus aureus: crosstalk between mammary epithelial cells and peripheral blood mononuclear cells, and how it governs immunity in the mammary gland of dairy cows |
| Grantee: | Fernando Nogueira de Souza |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |