A promising technology based on photoelectrocatalysis against Mycobacterium tuberculosis in water disinfection

Author(s)

Brugnera, Michelle Fernanda ^[1] ; Miyata, Marcelo ^[2] ; Zocolo, Guilherme Juliao ^{[3, 4]} ; Gonsalves Tessaro, Leticia Louize ^[1] ; Fujimura Leite, Clarice Queico ^[2] ; Boldrin Zanoni, Maria Valnice ^[4]

Affiliation

^[1] Univ Fed Mato Grosso, Inst Chem, Dept Chem, Cuiaba, MT - Brazil

^[2] UNESP, Dept Biol Sci, Araraquara, Fac Pharmaceut Sci Araraquara, Araraquara - Brazil

^[3] Embrapa Agroind Trop, Fortaleza, Ceara - Brazil

^[4] UNESP, Dept Analyt Chem, Inst Chem Araraquara, Araraquara - Brazil

• Document type: Journal article
• Source: ENVIRONMENTAL TECHNOLOGY; v. 42, n. 5, p. 743-752, FEB 23 2021.
• Web of Science Citations: 2

Abstract

Mycobacterium tuberculosis is highly infectious, persistent and has been detected in more than one quarter of the world's population. It is notoriously resistant to sterilization and disinfection procedures, largely due to an unusual hydrophobic cell wall and effective defense mechanisms against oxidative stress. This work shows an effective method to reduce M. tuberculosis quantity in water by using Ti/TiO2 nanotubes electrodes bare and coated with Ag nanoparticles by using photoelectrocatalytic oxidation process. The results have indicated 99.999% of inactivation of a solution spiked with standard and resistant strains of 1x10(4) CFU mL(-1) M. tuberculosis after 5 min of treatment at Ti/TiO2 photoanode in 0.05 mol L-1 Na2SO4 (pH 6) under applied potential of + 1.5 V versus Ag/AgCl and UV irradiation. The mycobacteria degradation was monitored by dissolved total organic carbon (TOC) removal, carbohydrate release, chromatography coupled to mass spectroscopy measurements and it is slightly superior to photocatalysis and photolysis processes. All the results corroborated with the complete inactivation and degradation of the byproducts generated during cell lysis. {[}GRAPHICS] . (AU)

• FAPESP's process: 09/09403-5 - Electroanalytical and chromatographic methods applied in the analysis of mycobacteria and photoeletrocatalytic disinfection of mycobacteria and candidas using electrodes of Ti/TiO2 and Ti/TiO2-Ag nanotubes
• Grantee: Michelle Fernanda Brugnera
• Support Opportunities: Scholarships in Brazil - Doctorate