Effect of maternal apical periodontitis on the final step of insulin signalling and inflammatory pathway in the adult male offspring of rats

Author(s)

Saori Tsosura, Thais Veronica ^{[1, 2]} ; de Lima Coutinho Mattera, Maria Sara ^[1] ; Chiba, Fernando Yamamoto ^[3] ; ascimento Carnevali, Ana Carolina ^[1] ; Belardi, Bianca Elvira ^{[1, 2]} ; Santos, Rodrigo Martins ^{[1, 2]} ; Angelo Cintra, Luciano Tavares ^[3] ; Lopes, Flavia Lombardi ^[4] ; Scaramele, Natalia Francisco ^[4] ; Matsushita, Doris Hissako ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Soc Brasileira Fisiol SBFis, Programa Posgrad Multicentr Ciencias Fisiol PPGMC, R Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Prevent & Restorat Dent, Aracatuba - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Vet Med, Dept Prod & Anim Hlth, Aracatuba - Brazil

• Document type: Journal article
• Source: International Endodontic Journal; v. 54, n. 11 SEP 2021.
• Web of Science Citations: 0

Abstract

Aim To evaluate the final step of insulin signalling, inflammatory pathway (related to the inhibition of insulin signalling), peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1 alpha) protein content and DNA methylation in the Slc2a4 gene promoter region in the skeletal muscle of adult male offspring of rats with apical periodontitis (AP) in a single tooth or in four teeth. Methodology Female Wistar rats were distributed into three groups: a control group, a group with one tooth with AP and a group with four teeth with AP. Thirty days after induction of AP, female rats from all groups were mated with healthy male rats. When male offspring reached 75 days of age, the following analyses were performed in the gastrocnemius muscle (GM): insulin-stimulated Akt serine and threonine phosphorylation status; NF-kappa B p50 and p65 subunits phosphorylation status; GLUT4, TNF-alpha and PGC-1 alpha protein content by Western blotting; GLUT4 and TNF-alpha gene expression by real-time polymerase chain reaction (PCR); and DNA methylation in the Slc2a4 gene promoter region by restriction digestion and real-time PCR. Analysis of variance was performed, followed by Tukey's post hoc test. p values Maternal AP in four teeth decreased insulin-stimulated Akt serine and threonine phosphorylation status, reduced GLUT4 gene expression and its protein content, and increased NF-kappa B p50 and p65 subunits phosphorylation status in the GM of adult offspring. There were no alterations in the parameters analysed in the GM of adult offspring of rats with AP in a single tooth. In addition, maternal AP did not affect TNF-alpha gene expression and its protein content, PGC-1 alpha protein content and DNA methylation in the Slc2a4 gene promoter region in the GM of adult offspring. Conclusions Maternal AP in four teeth was associated with impairment in the final step of insulin signalling in the GM of adult male offspring in rats. An increase in NF-kappa B activity may be involved in this decrease in insulin signalling. This study demonstrates the impact of maternal AP on the health of offspring, demonstrating the importance of maintaining adequate maternal oral health to prevent diseases in adult offspring in rats. (AU)

• FAPESP's process: 19/04182-2 - Assessment of the final steps of insulin signaling, inflammatory pathway, oxidative stress, epigenetic mechanism by DNA methylation and GLUT4 expression in the gastrocnemius muscle of adult rats, offspring of rats with Apical Periodontitis
• Grantee: Thaís Verônica Saori Tsosura
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/08706-3 - Evaluation of protein content and DNA methylation of GLUT4 in the gastrocnemius muscle of adult rats, offspring of rats with apical periodontitis
• Grantee: Ana Carolina Nascimento Carnevali
• Support Opportunities: Scholarships in Brazil - Scientific Initiation