Local and Systemic Inflammation and Oxidative Stress After a Single Bout of Maximal Walking in Patients With Symptomatic Peripheral Artery Disease

Author(s)

Andrade-Lima, Aluisio ^[1] ; Junior, Natan da Silva ^[1] ; Chehuen, Marcel ^[1] ; Miyasato, Roberto ^[1] ; Souza, Rodrigo W. A. ^[1] ; Leicht, Anthony S. ^[2] ; Brum, Patricia C. ^[1] ; de Oliveira, Edilamar M. ^[1] ; Wolosker, Nelson ^[3] ; Forjaz, Claudia L. M. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Av Prof Melo Moraes 65, BR-05508030 Sao Paulo - Brazil

^[2] James Cook Univ, Sport & Exercise Sdence, Townsville, Qld - Australia

^[3] Albert Einstein Israelite Hosp, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF CARDIOVASCULAR NURSING; v. 36, n. 5, p. 498-506, SEP-OCT 2021.
• Web of Science Citations: 1

Abstract

Objective The aim of this study was to assess the effects of a single bout of maximal walking on blood and muscle nitric oxide (NO) bioavailability, oxidative stress, and inflammation in symptomatic peripheral artery disease (PAD) patients. Methods A total of 35 men with symptomatic PAD performed a graded maximal exercise test on a treadmill (3.2 km/h, 2% increase in grade every 2 minutes). Plasma samples and gastrocnemius muscle biopsies were collected preexercise and postexercise for assessment of NO bioavailability (plasma NO and muscle, endothelial NO synthase), oxidative stress and antioxidant function (lipid peroxidation {[}LPO], catalase {[}CAT], and superoxide dismutase), and inflammation (interleukin-6, C-reactive protein, tumor necrosis factor-alpha, intercellular adhesion molecules, and vascular adhesion molecules). The effects of the walking exercise were assessed using paired t tests or Wilcoxon tests. Results After maximal walking, plasma NO and LPO were unchanged (P > .05), plasma CAT decreased, and all blood inflammatory markers increased (all P <= .05). In the disease-affected skeletal muscle, endothelial NO synthase, CAT, LPO, and all inflammatory markers increased, whereas superoxide dismutase decreased (all P <= .05). Conclusion In patients with symptomatic PAD, maximal exercise induces local and systemic impairments, which may play a key role in atherogenesis. Exercise strategies that avoid maximal effort may be important to reduce local and systemic damage and enhance clinical benefits. (AU)

• FAPESP's process: 15/13800-0 - Walking training in intermittent claudication: responses at rest and after maximal exercise
• Grantee: Cláudia Lúcia de Moraes Forjaz
• Support Opportunities: Regular Research Grants