Distribution, accumulation and speciation of selenium at the different growth stages of four garlic clones

Author(s)

Perez, Maria B. ^{[1, 2]} ; Lipinski, Victor M. ^[2] ; Fillipini, Maria F. ^[3] ; Chacon Madrid, Katherine ^[4] ; Zezzi Arruda, Marco A. ^[4] ; Wuilloud, Rodolfo G. ^{[1, 5]}

Affiliation

^[1] Consejo Nacl Invest Cient & Tecn CONICET, Padre J Contreras 1300, RA-5500 Mendoza - Argentina

^[2] Inst Tecnol Agropecuaria INTA, Estn Expt Consulta, Mendoza - Argentina

^[3] Univ Nacl Cuyo, Fac Ciencias Agr, Mendoza - Argentina

^[4] Univ Campinas Unicamp, Inst Chem, Grp Spectrometry Sample Preparat & Mechanizat GEP, Dept Analyt Chem, Campinas - Brazil

^[5] Univ Nacl Cuyo, Fac Ciencias Exactas & Nat, Lab Quim Analit Invest & Desarrollo QUIANID, Inst Interdisciplinario Ciencias Basicas ICB, Mendoza - Argentina

• Document type: Journal article
• Source: Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure; v. 38, n. 9, p. 1506-1519, SEP 2 2021.
• Web of Science Citations: 0

Abstract

Selenium (Se) is an essential micronutrient for humans. Garlic (Allium sativum L.) metabolises Se into important Se-amino acids like Se-methylselenocysteine (Se-MetSeCys), precursor of methylselenol, an active species for cancer prevention. Therefore, the Se accumulation and speciation in garlic were studied to evaluate their relations with growth stages and types of plant clones. Four garlic clones (Nieve INTA, Union FCA, Gostoso INTA and Rubi INTA) were fortified with a Se solution (169 g Se L-1). The association of Se to different molecular weight fractions was evaluated by size-exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP-MS) detection. Also, anion exchange chromatography (AEC-ICP-MS) was used for the determination of Se-amino acids, while their identification was performed by ESI-MS/MS. The Se was incorporated into high (7-5 kDa) and low (2-4 kDa) molecular weight fractions. The presence of Se-MetSeCys was observed mostly. Se-MetSeCys increased in bulbs to a maximum value but increased, then decreased, in leaves and roots. The Se-organic species were mostly found in bulbs in the last growth stage. Garlic showed a significant ability to accumulate and metabolise Se, specially, the red clones (Gostoso INTA and Rubi INTA). Also, this work suggests that this plant may become an attractive source of Se-amino acids with important biological properties. (AU)

• FAPESP's process: 18/25207-0 - Nanometallomics applied to soybean cultivation for studying the effects of metal-related nanoparticles
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants