| Full text | |
| Author(s): |
Casarin, Heitor Hussni
[1]
;
Mattos, Vicente Silva
[2]
;
de Castro Neto, Jarbas Caiado
[2]
;
Chinelatti, Michelle Alexandra
[1]
Total Authors: 4
|
| Affiliation: | [1] Cent Paulista Univ Ctr UNICEP Sao Carlos, Sch Dent, Sao Carlos - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | MICROSCOPY RESEARCH AND TECHNIQUE; v. 84, n. 10, p. 2399-2408, OCT 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Chemical composition of dental enamel has a great relationship with the prevention of caries. The objective of the present work was to evaluate the chemical and morphological changes of femtosecond laser-irradiated enamel with subablative parameters using Raman spectroscopy, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM). Bovine incisor teeth were used to obtain 30 enamel specimens (5 x 5 mm(2)). The chemical composition of the control sample was analyzed by Raman spectrometry to acquire the absorption spectrum, delimiting the areas under the carbonate and phosphate bands. This analysis was used to evaluate the change in the chemical composition of the sample after irradiation. The specimens were irradiated (IRR) with a Ti:Sapphire laser system (pulsed and focused modes, femtosecond regime 70 fs, average power of 1 W and exposure time of 15 s). After irradiation, the areas under the carbonate and phosphate absorption bands were delimited in each specimen. Raman spectrometry data were analyzed using Student's t-test (alpha = 5%). By comparing the spectra of the IRR and non-irradiated (NI) specimens, the results showed a significant increase in the area value for the phosphate peaks and a significant reduction in the area value for the carbonate peak and the carbonate:phosphate ratio. CLSM and SEM analyses did not reveal structural alterations in the subsurface nor morphological alterations in the IRR enamel surface, respectively. It was concluded that femtosecond laser irradiation using subablative parameters reduced the carbonate content and the carbonate/phosphate ratio without altering the structure and morphology of the dental enamel. (AU) | |
| FAPESP's process: | 19/21683-5 - Chemical and morphological alterations of dental enamel irradiated by femtosecond laser with sub-ablative parameters |
| Grantee: | Heitor Hussni Casarin |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |