MMP-2 and MMP-9 levels in plasma are altered and associated with mortality in COVID-19 patients

Author(s):Show less -

D'Avila-Mesquita, Carolina ^[1] ; Couto, Ariel E. S. ^[1] ; Campos, Ligia C. B. ^[1] ; Vasconcelos, Tauana F. ^[2] ; Michelon-Barbosa, Jessyca ^[1] ; Corsi, Carlos A. C. ^[1] ; Mestriner, Fabiola ^[1] ; Petroski-Moraes, Bruno C. ^[2] ; Garbellini-Diab, Maria J. ^[1] ; Couto, Daniel M. S. ^[1] ; Jordani, Maria C. ^[1] ; Ferro, Denise ^[3] ; Sbragia, Lourenco ^[4] ; Joviliano, Edwaldo E. ^[1] ; Evora, Paulo R. ^[3] ; Santana, Rodrigo de Carvalho ^[5] ; Martins-Filho, Olindo Assis ^[6] ; Polonis, Katarzyna ^[7] ; Menegueti, Mayra G. ^[8] ; Ribeiro, Mauricio S. ^[1] ; Auxiliadora-Martins, Maria ^[2] ; Becari, Christiane ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Div Vasc & Endovasc Surg, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Div Intens Care, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Div Cardiac Surg, Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Clin Med, Div Infect Dis, Ribeirao Preto - Brazil

^[6] FIOCRUZ Minas, Rene Rachou Inst, Oswaldo Cruz Fdn, Belo Horizonte - Brazil

^[7] Mayo Clin, Dept Lab Med & Pathol, Rochester, MN - USA

^[8] Univ Sao Paulo, Ribeirao Preto Nurse Med Sch, Ribeirao Preto - Brazil

• Document type: Journal article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 142, OCT 2021.
• Web of Science Citations: 0

Abstract

Respiratory symptoms are one of COVID-19 manifestations, and the metalloproteinases (MMPs) have essential roles in the lung physiology. We sought to characterize the plasmatic levels of matrix metalloproteinase-2 and 9 (MMP-2 and MMP-9) in patients with severe COVID-19 and to investigate an association between plasma MMP-2 and MMP-9 levels and clinical outcomes and mortality. MMP-2 and MMP-9 levels in plasma from patients with COVID-19 treated in the ICU (COVID-19 group) and Control patients were measured with the zymography. The study groups were matched for age, sex, hypertension, diabetes, BMI, and obesity profile. MMP-2 levels were lower and MMP-9 levels were higher in a COVID-19 group (p < 0.0001) compared to Controls. MMP-9 levels in COVID-19 patients were not affected by comorbidity such as hypertension or obesity. MMP-2 levels were affected by hypertension (p < 0.05), but unaffected by obesity status. Notably, hypertensive COVID-19 patients had higher MMP-2 levels compared to the non-hypertensive COVID-19 group, albeit still lower than Controls (p < 0.05). No association between MMP-2 and MMP-9 plasmatic levels and corticosteroid treatment or acute kidney injury was found in COVID-19 patients. The survival analysis showed that COVID-19 mortality was associated with increased MMP-2 and MMP-9 levels. Age, hypertension, BMI, and MMP-2 and MMP-9 were better predictors of mortality during hospitalization than SAPS3 and SOFA scores at hospital admission. In conclusion, a significant association between MMP-2 and MMP-9 levels and COVID-19 was found. Notably, MMP-2 and MMP-9 levels predicted the risk of in-hospital death suggesting possible pathophysiologic and prognostic roles. (AU)

• FAPESP's process: 18/23718-8 - Role of renin angiotensin system in abdominal aortic aneurysm
• Grantee: Christiane Becari
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 19/24369-0 - Renin-angiotensin system molecular analyses an in the development of in the Ang-II induced aneurysm in mice
• Grantee: Carolina D'Avila Mesquita
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 21/01195-6 - Evaluation of inflammatory mediators and nitric oxide in plasma of septic patients submitted to treatment with methylene blue
• Grantee: Bruno Cesar Petroski Moraes
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 19/21721-4 - Biochemical and molecular analysis of the renin-angiotensin system (RAS) components in the pathophysiology of abdominal aortic aneurysm (AAA) in patients undergoing AAA correction surgery at HCRP-USP
• Grantee: Jéssyca Michelon Barbosa
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 20/09999-4 - Biochemical characterization of angiotensin (Ang) I without plasma and aorta of C57Bl / 6 and ELA-2KO mice with abdominal aortic aneurysm (AAA) induced by the infusion of II
• Grantee: Lígia Cristina Borges Campos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/11485-1 - Evaluation of cost-effectiveness and thrombotic markers of the varice techniques' treatment in the public health system
• Grantee: Mauricio Serra Ribeiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/03308-0 - Aorta morphological analysis of ELA-2KO mice with abdominal aortic aneurysm induced by the infusion of Angiotensin II
• Grantee: Ariel Emiliano Souza do Couto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation