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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacerein

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Author(s):
de Oliveira, Salmo Azambuja [1] ; Cerri, Paulo Sergio [2] ; Sasso-Cerri, Estela [2]
Total Authors: 3
Affiliation:
[1] Univ Fed Sao Paulo, Dept Morphol & Genet, Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Dent Sch, Dept Morphol Genet Orthodont & Pediat Dent, Lab Histol & Embryol, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Histochemistry and Cell Biology; v. 156, n. 6 SEP 2021.
Web of Science Citations: 1
Abstract

The role of cytokines in testicular function under normal conditions has not been completely understood. Here, we evaluated testicular macrophages (TM), steroidogenesis by Leydig cells (LC) and seminiferous tubules integrity in cytokines-deficient rat testes induced by diacerein, an anti-inflammatory drug that inhibits interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). Male rats received daily 100 mg/kg of diacerein (DIAG; n=8) or saline (CG; n=8) for 30 days. Serum testosterone (T) levels were measured and the seminiferous tubule (ST) area, epithelial area (EA), frequency of damaged ST and number of Sertoli cells (SC) were evaluated. TUNEL method and immunoreactions for detection of pro-IL-1 beta, TNF-alpha, steroidogenic acute regulatory protein (StAR), 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD), androgen receptor (AR) and scavenger receptor for hemoglobin-haptoglobin complexes (CD163), a TM marker, were performed. Testicular AR, 17 beta-HSD and IL-1p levels were detected by Western blot. Data were submitted to Student t test (p <= 0.05). In DIAG, T and testicular AR, 17 beta-HSD and IL-1 beta levels decreased significantly (p < 0.05). The number of TUNEL-positive interstitial cells increased and LC showed weak StAR, 17 beta-HSD and AR immunoexpression in association with reduced IL-1 beta immunoexpression and number of CD163-positive TM in the interstitial tissue from diacerein-treated rats. Numerous damaged ST were found in DIAG, and reduction in the EA were associated with germ cells death. Moreover, the number of SC reduced and weak AR and TNF-alpha immunoexpression was observed in SC and germ cells, respectively. The cytokines deficiency induced by diacerein impairs TM, LC and spermatogenesis, and points to a role of IL-1 beta in steroidogenesis under normal conditions. In the ST, the weak AR and TNF-alpha immunoexpression in SC and germ cells, respectively, reinforces the idea that TNF-alpha plays a role in the SC androgenic control. (AU)

FAPESP's process: 17/19829-6 - EFFECT OF VENLAFAXINE ON THE MALE REPRODUCTIVE HISTOPHYSIOLOGY AND SPERM PARAMETERS OF ADULT RATS
Grantee:Estela Sasso Cerri
Support Opportunities: Regular Research Grants