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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crystal Structure of a Sucrose-6-phosphate Hydrolase from Lactobacillus gasseri with Potential Applications in Fructan Production and the Food Industry

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Author(s):
de Lima, Mariana Z. T. [1] ; de Almeida, Leonardo R. [1] ; Mera, Alain M. [1] ; Bernardes, Amanda [1] ; Garcia, Wanius [2] ; Muniz, Joao R. C. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, BR-13563120 Sao Carlos, SP - Brazil
[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas CCNH, BR-09210580 Santo Andre, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Agricultural and Food Chemistry; v. 69, n. 35, p. 10223-10234, SEP 8 2021.
Web of Science Citations: 0
Abstract

Fructooligosaccharides (FOSs) are polymers of fructose with a prebiotic activity because of their production and fermentation by bacteria that inhabit the gastrointestinal tract and are widely used in the industry and new functional foods. Lactobacillus gasseri stands out as an important homofermentative microorganism related to FOS production, and its potential applications in the industry are undeniable. In this study, we report the production and characterization of a sucrose-6-phosphate hydrolase from L. gasseri belonging to the GH32 family. Apo-LgAs32 and LgAs32 complexed with beta-D-fructose structures were determined at a resolution of 1.94 and 1.84 angstrom, respectively. The production of FOS, fructans, 1-kestose, and nystose by the recombinant LgAs32, using sucrose as a substrate, shown in this study is very promising. When compared to its homologous enzyme from Lactobacillus reuteri, the production of 1-kestose by LgAs32 is increased; thus, LgAs32 can be considered as an alternative in fructan production and other industrial applications. (AU)

FAPESP's process: 16/09152-6 - Structural and functional studies of cellulosomal thermophilic glycoside hydrolases involved on lignocellulosic biomass hydrolysis
Grantee:Leonardo Rodrigues de Almeida
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/50897-0 - INCT 2014: Open-acess Medicinal Chemistry Centre (OpenMedChem)
Grantee:Katlin Brauer Massirer
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/17275-3 - Studies of the mode of action of two lytic polysaccharide monooxygenases from insect (order: isoptera): molecular structure, bioinorganic chemistry and biotechnological applications
Grantee:Wanius José Garcia da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 17/16291-5 - Structural and biophysical characterization of thermophilic enzymes prospected from the fungus Thielavia terrestris for biomass degradation and biotechnological products generation
Grantee:João Renato Carvalho Muniz
Support Opportunities: Regular Research Grants