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Structural and functional studies of cellulosomal thermophilic glycoside hydrolases involved on lignocellulosic biomass hydrolysis

Grant number: 16/09152-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2016
Effective date (End): November 30, 2020
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:João Renato Carvalho Muniz
Grantee:Leonardo Rodrigues de Almeida
Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil


The drop of petroleum reserves, the growing energy demand from emerging countries, and the eminent necessity for reducing carbon dioxide emissions indicate the importance for the pursuit of new sources of renewable energy. However, such sources must have a real character of effective replacement and not only be complementary to the existing resources. Thus, the bioethanol production by hydrolysis of lignocellulosic biomass, such as sugarcane bagasse, has received considerable attention. Due to its potential for development and cost reduction, the enzymatic cellulose hydrolysis can be the key factor for second generation bioethanol production at competitive cost in long term. Thermophilical bacteria, such as Clostridium thermocellum, produces an extracellular multi-enzyme complex named cellulosome, which has been demonstrating a very high ability for an efficient degradation of cellulosic biomass, in particular the crystalline portion of cellulose. The main objective of this research project is to characterize the cellulosomal ²-glucosidases enzymes from C. thermocellum of different glycoside hydrolases (GHs) families, applying structural and biophysical analyses. Considering that purpose, we shall conduct comparative studies, specifically from GH1 and GH3 glycoside hydrolases families. Therefore, it is intended to understand the enzymatic mechanisms of cellulosomal ²-glucosidase, which can form the basis for obtaining enzymes with improved performance on biomass depolymerisation.

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE LIMA, MARIANA Z. T.; DE ALMEIDA, LEONARDO R.; MERA, ALAIN M.; BERNARDES, AMANDA; GARCIA, WANIUS; MUNIZ, JOAO R. C.. Crystal Structure of a Sucrose-6-phosphate Hydrolase from Lactobacillus gasseri with Potential Applications in Fructan Production and the Food Industry. Journal of Agricultural and Food Chemistry, v. 69, n. 35, p. 10223-10234, . (16/09152-6, 14/50897-0, 17/17275-3, 17/16291-5)
SOUZA, MATHEUS S.; DA SILVA, CECILIA C. P.; ALMEIDA, LEONARDO R.; DINIZ, LUAN F.; ANDRADE, MARCELO B.; ELLENA, JAVIER. Protonation of inorganic 5-Fluorocytosine salts. Journal of Molecular Structure, v. 1161, p. 412-423, . (13/03487-8, 16/09152-6, 15/25694-0)
CUSTODIO, JEAN M. F.; GOTARDO, FERNANDO; VAZ, WESLEY F.; D'OLIVEIRA, GIULIO D. C.; DE ALMEIDA, LEONARDO R.; FONSECA, RUBEN D.; COCCA, LEANDRO H. Z.; PEREZ, CARIDAD N.; OLIVER, ALLEN G.; DE BONI, LEONARDO; et al. Benzenesulfonyl incorporated chalcones: Synthesis, structural and optical properties. Journal of Molecular Structure, v. 1208, . (18/11283-7, 16/20886-1, 16/09152-6)
CAMARGO, SUELEN; MULINARI, EVANDRO J.; DE ALMEIDA, LEONARDO R.; BERNARDES, AMANDA; PRADE, ROLF A.; GARCIA, WANIUS; SEGATO, FERNANDO; MUNIZ, JOAO R. C.. Functional and structural characterization of an alpha-L-arabinofuranosidase from Thermothielavioides terrestris and its exquisite domain-swapped beta-propeller fold crystal packing. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1868, n. 12, p. 12-pg., . (17/16291-5, 16/09152-6, 14/18714-2, 14/50897-0, 19/22284-7, 17/50025-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALMEIDA, Leonardo Rodrigues de. Structural and functional studies of glycoside hydrolases from thermophilic microorganisms. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.

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