| Full text | |
| Author(s): |
de Lima, Mariana Z. T.
[1]
;
de Almeida, Leonardo R.
[1]
;
Mera, Alain M.
[1]
;
Bernardes, Amanda
[1]
;
Garcia, Wanius
[2]
;
Muniz, Joao R. C.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, BR-13563120 Sao Carlos, SP - Brazil
[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas CCNH, BR-09210580 Santo Andre, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Agricultural and Food Chemistry; v. 69, n. 35, p. 10223-10234, SEP 8 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Fructooligosaccharides (FOSs) are polymers of fructose with a prebiotic activity because of their production and fermentation by bacteria that inhabit the gastrointestinal tract and are widely used in the industry and new functional foods. Lactobacillus gasseri stands out as an important homofermentative microorganism related to FOS production, and its potential applications in the industry are undeniable. In this study, we report the production and characterization of a sucrose-6-phosphate hydrolase from L. gasseri belonging to the GH32 family. Apo-LgAs32 and LgAs32 complexed with beta-D-fructose structures were determined at a resolution of 1.94 and 1.84 angstrom, respectively. The production of FOS, fructans, 1-kestose, and nystose by the recombinant LgAs32, using sucrose as a substrate, shown in this study is very promising. When compared to its homologous enzyme from Lactobacillus reuteri, the production of 1-kestose by LgAs32 is increased; thus, LgAs32 can be considered as an alternative in fructan production and other industrial applications. (AU) | |
| FAPESP's process: | 16/09152-6 - Structural and functional studies of cellulosomal thermophilic glycoside hydrolases involved on lignocellulosic biomass hydrolysis |
| Grantee: | Leonardo Rodrigues de Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/50897-0 - INCT 2014: Open-acess Medicinal Chemistry Centre (OpenMedChem) |
| Grantee: | Katlin Brauer Massirer |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/17275-3 - Studies of the mode of action of two lytic polysaccharide monooxygenases from insect (order: isoptera): molecular structure, bioinorganic chemistry and biotechnological applications |
| Grantee: | Wanius José Garcia da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/16291-5 - Structural and biophysical characterization of thermophilic enzymes prospected from the fungus Thielavia terrestris for biomass degradation and biotechnological products generation |
| Grantee: | João Renato Carvalho Muniz |
| Support Opportunities: | Regular Research Grants |