Influence of interleukin 17 A and 17 F polymorphisms in keratoconus

Author(s)

Gomes, Isabela Bronchtein ^[1] ; Ayo, Christiane Maria ^[1] ; Lopes, Alessandro Garcia ^[2] ; Kumano, Laurie Sayuri ^[1] ; de Faria Junior, Geraldo Magela ^[1] ; de Almeida Jr, Gildasio Castello ; Castiglioni, Lilian ^{[1, 2]} ; de Mattos, Luiz Carlos ^[1] ; Brandao, Cinara Cassia ^[1]

Affiliation

^[1] Fac Med Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Lab Immunogenet, Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Paulo State Univ Julio de Mesquista Filho UNE, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: MOLECULAR BIOLOGY REPORTS; v. 48, n. 11 SEP 2021.
• Web of Science Citations: 0

Abstract

Background Until a few years ago, keratoconus was defined as a noninflammatory degenerative disease. However, recent studies have shown that the altered balance between inflammatory cytokines, proteases, and protease inhibitors, as well as free radicals and oxidants, have a crucial role in the pathogenesis of this disease. The aim of this study is to investigate whether interleukin 17 A G197A (rs2275913) and interleukin 17 F T7488C (rs763780) polymorphisms are associated with keratoconus in patients from a population of the northwestern region of the State of Sao Paulo, Brazil. Methods and Results 35 patients and 61 controls were enrolled. Genotyping of interleukin 17 A G197A and interleukin 17 F T7488C polymorphisms was carried out using the polymerase chain reaction-restriction fragment length polymorphism technique. Statistical analyses were conducted using the chi-square test, and an odds ratio with a 95% confidence interval was also calculated to evaluate the association between polymorphisms and disease. Evaluating interleukin 17 F T7488C, we found that the TT genotype is associated as a risk factor for keratoconus (P = 0.04; OR = 3.01; CI 1.11-8.14). As for evaluating interleukin 17 A G197A, the allele and genotype frequencies between patients and controls were compared and no statistically significant differences were found. Conclusions Our data showed that the interleukin 17 F T7488C polymorphisms may exert an influence in keratoconus. (AU)

• FAPESP's process: 15/17226-7 - Biomarkers in ocular diseases
• Grantee: Gildásio Castello de Almeida Júnior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/09448-8 - Expression of miRNAs in ocular Toxoplasmosis
• Grantee: Geraldo Magela de Faria Junior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/16622-4 - Association of the MICA-129 polymorphism with the development of keratoconus in a region North-west of the State of São Paulo
• Grantee: Laurie Sayuri Kumano
• Support Opportunities: Scholarships in Brazil - Scientific Initiation