ioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio

Author(s):Show less -

Viana, Natalia Prudencio ^{[1, 2]} ; Menezes da Silva, Lais Conceicao ^[3] ; Portruneli, Natalia ^{[1, 2]} ; Soares, Michelly Pereira ^{[2, 4]} ; Cardoso, Israel Luz ^{[2, 4]} ; Ines Bonansea, Rocio ^[5] ; Goulart, Bianca Veloso ^[6] ; Montagner, Cassiana Carolina ^[6] ; Gaeta Espindola, Evaldo Luiz ^[3] ; Alberto Wunderlin, Daniel ^[7] ; Fernandes, Marisa Narciso ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Carlos, Programa Posgrad Ecol & Recursos Nat, Rodovia Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos UFSCar, Dept Ciencias Fisiol, Rodovia Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Escola Engn Sao Carlos NEEA CRHEA SHS, Programa Posgrad Ciencias Engn Ambiental, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos, Programa Interinst Posgrad Ciencias Fisiol, Univ Estadual Sao Paulo, Rodovia Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Nacl Cordoba, Fac Ciencias Quim, Cordoba - Argentina

^[6] Univ Campinas UNICAMP, Inst Quim, BR-13083970 Campinas, SP - Brazil

^[7] Consejo Nacl Invest Cient & Tecn, Inst Ciencias & Tecnol Alimentos Cordoba, Cordoba - Argentina

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. 29, n. 8 SEP 2021.
• Web of Science Citations: 1

Abstract

The insecticide fipronil and the herbicide 2,4-D are the most applied pesticides in sugarcane crops leading to aquatic contamination. The whole-body bioconcentration of fipronil and 2,4-D, single and in mixture, was evaluated in Danio rerio after 96-h exposure. The activities of catalase (CAT) and glutathione S-transferase(GST) in whole body and in the gills and the acetylcholinesterase (AChE) in muscle were determined. The gill histopathology and the morphology of the pavement (PVC) and the mitochondria-rich(MRC) cells at gill surface were analyzed. Bioconcentration occurred after exposure to fipronil (2.69 L kg(-1)) and 2,4-D (1.73 L kg(-1)) single and in mixture of fipronil (3.10 L kg(-1)) and 2,4-D (1.27 L kg(-1)). Whole-body CAT activity was unchanged, and its activity decreased in the gills after exposure to fipronil and increased after exposure to 2,4-D and mixture. GST and AChE increased after single exposure to each pesticide and mixture of both. Fish exposed to mixture increased the MRC fractional area (MRCFA) which suggested possible ionic regulation disturbance and reduced the microridge of the PVC surface. Synergistic interactions occurred in the CAT activity and MRCFA after exposure to mixture of pesticides. The results indicate that the recommended application dose of fipronil and 2,4-D, single or in mixture, for sugarcane crops affects this fish species altering its homeostasis. (AU)

• FAPESP's process: 15/18790-3 - Environmental effects of the pasture-sugarcane conversion and pasture intensification
• Grantee: Luiz Antonio Martinelli
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants