Antimicrobial activity against Microcystis aeruginosa and degradation of microcystin-LR by bacteria isolated from Antarctica

Author(s)

Sosa Benegas, Gabriela Rocio ^{[1, 2]} ; Fernandes Bernal, Suzan Prado ^[1] ; de Oliveira, Valeria Maia ^[3] ; Zambrano Passarini, Michel Rodrigo ^[1]

Affiliation

^[1] UNILA Univ Fed Integracao Latino Amer, Lab Biotecnol Ambiental, Av Taiquinio Joslin Santos, 1000 Jd Univ, BR-85870650 Foz Do Iguacu, PR - Brazil

^[2] Div Embalse MARR CE, ITAIPU BINACL, Estacien Acuicultura, Labs Ecol, Supercarretera Itaipu, Km 16-5, Hernandarias - Paraguay

^[3] CPQBA UNICAMP Div Recursos Microbianos, Rua Alexandre Caselatto 999, CP 6171, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. 28, n. 37, p. 52381-52391, OCT 2021.
• Web of Science Citations: 0

Abstract

Cyanobacteria massive proliferations are common in freshwater bodies worldwide, causing adverse effects on aquatic ecosystems and public health. Numerous species develop blooms. Most of them correspond to the toxic microcystin-producing cyanobacterium Microcystis aeruginosa. Microorganisms recovered from Antarctic environment can be considered an unexploited source of antimicrobial compounds. Data about their activity against cyanobacteria are scant or inexistent. This study aimed to evaluate the capacity of Antarctic bacteria to inhibit the proliferation of M. aeruginosa BCPUSP232 and to degrade microcystin-LR (MC-LR). Cell-free extracts of seventy-six bacterial strains were initially tested for antimicrobial activity. Unidentified (UN) strains 62 and ES7 and Psychromonas arctica were able to effectively lyse M. aeruginosa. Eight strains showed MIC ranging from 0.55 to 3.00 mg mL(-1), with ES7 showing the best antimicrobial activity. Arthrobacter sp. 443 and UN 383 were the most efficient in degrading MC-LR, with 24.87 and 23.85% degradation, respectively. To our knowledge, this is the first report of antimicrobial and MC-LR degradation activities by Antarctic bacteria, opening up perspectives for their future application as an alternative or supporting approach to help mitigate cyanobacterial blooms. (AU)

• FAPESP's process: 16/05640-6 - Multi-omics applied to the understanding and exploitation of Antarctic microbiomes
• Grantee: Valeria Maia Merzel
• Support Opportunities: Regular Research Grants