| Full text | |
| Author(s): Show less - |
de Santana, C. David
[1]
;
Parenti, Lynne R.
[1]
;
Dillman, Casey B.
[2]
;
Coddington, Jonathan A.
[3]
;
Bastos, Douglas A.
[4]
;
Baldwin, Carole C.
[1]
;
Zuanon, Jansen
[5]
;
Torrente-Vilara, Gislene
[6]
;
Covain, Raphael
[7]
;
Menezes, Naercio A.
[8]
;
Datovo, Alessio
[8]
;
Sado, T.
[9]
;
Miya, M.
[9]
Total Authors: 13
|
| Affiliation: | [1] Smithsonian Inst, Div Fishes, Dept Vertebrate Zool, Natl Museum Nat Hist, MRC 159, POB 37012, Washington, DC 20013 - USA
[2] Cornell Univ, Museum Vertebrates, Dept Ecol & Evolutionary Biol, Ithaca, NY 14850 - USA
[3] Smithsonian Inst, Global Genome Initiat, Natl Museum Nat Hist, POB 37012, Washington, DC 20013 - USA
[4] Inst Nacl de Pesquisas da Amazonia, Programa Posgrad Ciencias Biol BADPI, Manaus, Amazonas - Brazil
[5] Inst Nacl de Pesquisas da Amazonia, Coordenacao Biodiversidade, Manaus, Amazonas - Brazil
[6] Univ Fed Sao Paulo, Inst Mar, Campus Baixada Santista, Santos, SP - Brazil
[7] Museum Nat Hist, Dept Herpetol & Ichthyol, Route Malagnou 1, POB 6434, CH-1211 Geneva 6 - Switzerland
[8] Univ Sao Paulo MZUSP, Museu Zool, Ave Nazare 481, BR-04263000 Sao Paulo, SP - Brazil
[9] Nat Hist Museum & Inst, Chuo Ku, Chiba 2608682 - Japan
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 11, n. 1 SEP 13 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Ichthyological surveys have traditionally been conducted using whole-specimen, capture-based sampling with varied but conventional fishing gear. Recently, environmental DNA (eDNA) metabarcoding has emerged as a complementary, and possible alternative, approach to whole-specimen methodologies. In the tropics, where much of the diversity remains undescribed, vast reaches continue unexplored, and anthropogenic activities are constant threats; there have been few eDNA attempts for ichthyological inventories. We tested the discriminatory power of eDNA using MiFish primers with existing public reference libraries and compared this with capture-based methods in two distinct ecosystems in the megadiverse Amazon basin. In our study, eDNA provided an accurate snapshot of the fishes at higher taxonomic levels and corroborated its effectiveness to detect specialized fish assemblages. Some flaws in fish metabarcoding studies are routine issues addressed in natural history museums. Thus, by expanding their archives and adopting a series of initiatives linking collection-based research, training and outreach, natural history museums can enable the effective use of eDNA to survey Earth's hotspots of biodiversity before taxa go extinct. Our project surveying poorly explored rivers and using DNA vouchered archives to build metabarcoding libraries for Neotropical fishes can serve as a model of this protocol. (AU) | |
| FAPESP's process: | 16/19075-9 - Diversity and evolution of Gymnotiformes (Teleostei, Ostariophysi) |
| Grantee: | Naercio Aquino Menezes |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/07910-0 - Javari River basin in the context of "Amazon fish and climate changes - AMAZONFISH" |
| Grantee: | Gislene Torrente Vilara |
| Support Opportunities: | Regular Research Grants |