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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immobilization of a peroxidase from Moringa oleifera Lam. roots (MoPOX) on chitosan beads enhanced the decolorization of textile dyes

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Author(s):
Lopes, Larissa Alves [1] ; Dias, Lucas Pinheiro [2] ; Silva da Costa, Helen Paula [1] ; da Silva Neto, Joao Xavier [1] ; Morais, Eva Gomes [1] ; Abreu de Oliveira, Jose Tadeu [1] ; Vasconcelos, Ilka Maria [1] ; Bezerra de Sousa, Daniele de Oliveira [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Ceara, Dept Bioquim & Biol Mol, BR-60451970 Fortaleza, Ceara - Brazil
[2] Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Process Biochemistry; v. 110, p. 129-141, NOV 2021.
Web of Science Citations: 0
Abstract

The textile industry is essential, but it is also responsible for causing environmental problems, particularly the discharge of dyes. In this context, this study aimed to immobilize a previously purified peroxidase, called MoPOX, on glutaraldehyde-activated chitosan beads in order to improve the potential for textile dye decolorization. The chitosan beads were activated with 8% glutaraldehyde for 1 h, and the immobilization was performed at 30 degrees C, pH 5.2 for 4 h. Scanning Electron Microscopy (SEM) analyses were used to observe the differences in the chitosan beads after immobilization. The optimum temperature dropped from 70 degrees C to 30 degrees C after immobilization, but immobilized MoPOX demonstrated excellent heat stability. The optimum pH remained 5.2, while the apparent kinetic constant value (Km) of immobilized MoPOX (14.67 mM) was lower in comparison to free MoPOX (46.8 mM). The immobilized enzyme showed improved activity after long storage times, and it could retain 40 % of its original activity even after 5 cycles. The potential for decolorization of different textile dyes was considerably enhanced after immobilization, reaching more than 80 %. Also, MoPOX showed no toxicity towards Artemia salina. Overall, the findings point to the promising potential of using immobilized MoPOX as a biocatalyst in a variety of biotechnological applications. (AU)

FAPESP's process: 20/03998-6 - Effect of the interaction of antimicrobial peptides on the structuring of the lipid bilayer
Grantee:Lucas Pinheiro Dias
Support Opportunities: Scholarships in Brazil - Post-Doctoral