Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid

Full text
Author(s):
Mahmoud, Ramez H. [1] ; Biguetti, Claudia C. [2] ; Simionato, Gustavo B. [1] ; Custodio, Isabela C. [3] ; Silva, Raquel B. P. [1] ; Duarte, Marco A. H. [4] ; Faverani, Leonardo P. [5] ; Ervolino, Edilson [1] ; Fakhouri, Walid D. [6] ; Matsumoto, Mariza A. [1]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ, Sch Dent, Dept Basic Sci, UNESP, Aracatuba, SP - Brazil
[2] Univ Texas Dallas, Dept Bioengn, Richardson, TX 75083 - USA
[3] Ctr Univ Sagrad Coracao, Dept Hlth Sci, Bauru, SP - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, FOB USP, Bauru, SP - Brazil
[5] Sao Paulo State Univ, Sch Dent, Dept Surg, UNESP, Aracatuba, SP - Brazil
[6] Sao Paulo State Univ, Sch Dent, Dept Surg, Ctr Craniofacial Res, UNESP, Houston, TX - USA
Total Affiliations: 6
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 11, n. 1 OCT 1 2021.
Web of Science Citations: 0
Abstract

This study investigated the role 5-lypoxigenase (5-LO) on alveolar socket healing in aged female mice treated with zoledronic acid (ZL). Forty 129/Sv female mice (64-68 weeks old), 20 wild type (WT) and 20 5-LO knockout (5LOKO) were equally distributed according to ZL treatment: WT Control, WT ZL, 5LOKO Control, and 5LOKO ZL. ZL groups were treated with an intraperitoneal injection of 250 mu g/Kg of ZL, while controls were treated with saline. Treatments were administered once a week, starting four weeks before surgery for tooth extraction and until 7 and 21 days post-surgery. Mice were euthanized for a comprehensive microscopic analysis (microCT, histomorphometry and immunohistochemistry). WT ZL mice presented intense inflammatory infiltrate (7 days), delayed bone formation (21 days), reduced collagenous matrix quality, and a deficiency in Runx-2 + , TRAP + , and macrophages as compared to controls. 5LOKO ZL animals presented decreased number of Runx-2 + cells in comparison to 5LOKO Control at 7 days, but no major changes in bone healing as compared to WT or 5LOKO mice at 21 days. The knockout of 5LO favored intramembranous bone healing in aged female mice, with a direct impact on inflammatory response and bone metabolism on the development of ONJ-like lesions. (AU)

FAPESP's process: 19/16067-3 - Influence of nitrogen-containing bisphosphonates (n-BPs) on inflammatory and endocrine factors: basis for the comprehension of osteonecrosis of the jaws related to nBPs pathophysiology
Grantee:Mariza Akemi Matsumoto
Support Opportunities: Regular Research Grants
FAPESP's process: 18/19409-0 - Alveolar bone repair post tooth extraction of 129/SV- WT and 129/Sv 5LOKO elderly female mice treated with high-potency bisphosphonate
Grantee:Isabela do Carmo Custodio
Support Opportunities: Scholarships in Brazil - Scientific Initiation