| Full text | |
| Author(s): |
A. Lillo
[1]
;
F. Carvajal-Caiconte
[2]
;
W. Vital
[3]
;
P. I. Silva Junior
[4]
;
E. J. Paredes-Gamero
[5]
;
M. Alvear
[6]
;
A. Miranda
[7]
Total Authors: 7
|
| Affiliation: | [1] Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia - Brasil
[2] Universidad de La Frontera. Departamento de Ciencias Químicas y Recursos Naturales - Chile
[3] Universidade Federal de São Paulo. Departamento de Biologia Química - Brasil
[4] Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular. Centro de Pesquisa, Inovação e Difusão - Brasil
[5] Universidade Federal de Mato Grosso do Sul. Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição - Brasil
[6] Universidad de La Frontera. Departamento de Ciencias Químicas y Recursos Naturales - Chile
[7] Universidade Federal de São Paulo. Departamento de Biofísica - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Biology; v. 83, 2021-09-06. |
| Abstract | |
Abstract Persea lingue Ness is a tree species that lives mainly in temperate forests of south-central Chile. Its leaves are used in ethnomedicine, the fruit is a drupe similar to that of the avocado and has not been studied. The aim of this study was to determine the cytotoxicity in leukemia cell and antibacterial activity, along with some chemical content characteristics of P. lingue fruit and leaf extracts. The antibacterial activity was determined by the inhibition of bacterial growth in liquid medium assay against Gram-positive and Gram-negative bacteria. The leukemia cell lines Kasumi-1 and Jurkat were used to evaluate the cytotoxic activity by using propidium iodide and AlamarBlue assays. Total phenolic, flavonoid, condensed tannin, alkaloid and lipid contents were evaluated in the fruit and in the leaf extracts. The antioxidant activity of both extracts were also elavaluated. Leaf extract presented the highest content of total phenols, condensed tannins and flavonoids, and also the highest antioxidant activity. While the fruit extract has a higher amount of lipids and alkaloids and the high antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus megaterium and Micrococcus luteus. The leaf extract only showed activity against M. luteus. Concerning the cytotoxic activity, only the fruit extract showed cytotoxicity against the cell lines Jurkat and Kasumi-1. P. lingue fruit extract is a potential source of biologically active molecules for the development of new drugs to be used in some types of leukemia, as well as antibacterial agent. (AU) | |
| FAPESP's process: | 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling |
| Grantee: | Hugo Aguirre Armelin |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |