Effect of non-steroidal anti-inflammatory drugs on pulpal and periapical inflammation induced by lipopolysaccharide

Author(s)

Ribeiro-Santos, Fernanda Regina ^{[1, 2]} ; Arnez, Maya Fernanda Manfrin ^[2] ; de Carvalho, Marcio Santos ^[2] ; da Silva, Raquel Assed Bezerra ^[2] ; Politi, Marilia Pacifico Lucisano ^[2] ; de Queiroz, Alexandra Mussolino ^[2] ; Nelson-Filho, Paulo ^[2] ; da Silva, Lea Assed Bezerra ^[2] ; Faccioli, Lucia Helena ^[3] ; Paula-Silva, Francisco Wanderley Garcia ^{[3, 2]}

Affiliation

^[1] Univ Pernambuco, Arco Verde, PE - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Ave Cafe, S-N, BR-14040904 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Sao Paulo - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 11, p. 6201-6209, NOV 2021.
• Web of Science Citations: 0

Abstract

Objectives The objective of this study was to evaluate the effect of non-steroidal anti-inflammatory drugs (NSAIDs) in controlling pulpal and periapical inflammation in vivo as a potential coadjutant systemic therapy for pulpitis. Materials and methods A suspension containing E. coli lipopolysaccharide (LPS; 1.0 mu g/mu L) was inoculated into the pulp chamber of the first molars of C57BL/6 mice (n = 72), and the animals were treated daily with indomethacin or celecoxib throughout the experimental periods. After 7, 14, 21, and 28 days, the tissues were removed for histopathological, histoenzymology, histometric, and immunohistochemical evaluation. Results Inoculation of LPS into the pulp chamber induced the synthesis of the enzyme cyclooxygenase-2 (COX-2) in dental pulp and periapical region. Indomethacin and celecoxib treatment changed the profile of inflammatory cells recruited to dental pulp and to the periapex, which was characterized by a higher mononuclear cell infiltrate, compared to LPS inoculation alone which recruited a higher amount of polymorphonuclear neutrophils. Administration of indomethacin for 28 days resulted in the development of apical periodontitis and increased osteoclast recruitment, unlike celecoxib. Conclusions NSAIDs indomethacin and celecoxib changed the recruitment of inflammatory cells to a mononuclear profile upon inoculation of LPS into the pup chamber, but indomethacin enhanced periapical bone loss whereas celecoxib did not. (AU)

• FAPESP's process: 12/08421-2 - Investigation of the role of cyclooxygenase-2 in periapical bone resorption induced by bacterial lipopolysaccharide
• Grantee: Fernanda Regina Ribeiro Santos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/17611-4 - Mechanisms involved in the regulation of 5-lipoxygenase pathway in experimentally-induced apical periodontitis
• Grantee: Francisco Wanderley Garcia de Paula e Silva
• Support Opportunities: Research Grants - Young Investigators Grants