Both spontaneous vaginal delivery and elective caesarean section influence neonatal redox status in dogs

Author(s)

de Almeida, Leticia Lima ^[1] ; de Abreu, Renata Azevedo ^[1] ; Brito, Maira Morales ^[1] ; Gardes, Thayna Pantoja ^[1] ; Flores, Renato Bueno ^[1] ; da Rosa Filho, Roberto Rodrigues ^[1] ; Vannucchi, Camila Infantosi ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Rua Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: VETERINARY RECORD; NOV 2021.
• Web of Science Citations: 0

Abstract

Background The influence of birth mode on redox balance is unknown in dogs. We aimed to compare antioxidant activity and oxidative stress of puppies born by elective c-section or vaginal delivery. Methods Twenty-one bitches were elected for spontaneous vaginal delivery (n = 10) and elective cesarean section (n = 11) groups, considering only the first and last puppies born. Neonatal oxygen saturation, lactatemia, superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and total antioxidant capacity; marker of oxidative stress (thiobarbituric acid reactive substances {[}TBARS]) and protein oxidation were analysed at birth, 1 h, 12 h, 24 h and 72 h. Results C-section newborns had lower oximetry at birth and 1 h. Lactatemia was higher in the last vaginal delivery puppies and higher at birth, and 1 h. Regardless of birth mode, last puppies had hyperlactatemia at birth. Vaginal delivery puppies had higher TBARS, while protein oxidation was higher in c-section, mostly in last puppies. C-section puppies had higher GPx activity, whereas GSH concentration decreased overtime. Surgically born puppies had lowering SOD between 12 h and 72 h. Conclusions Vaginal delivery enhances lipid peroxidation, counteracted mostly by GPx. Conversely, c-section triggers protein oxidation, acutely scavenged by SOD and GSH, thus puppies are less exposed to the negative consequences of oxidative stress. (AU)

• FAPESP's process: 17/11844-6 - Small animal perinatology challenges: the influence of racial conformation on maternal adaptation to pregnancy and the influence of the obstetric condition on feto-neonatal transition period.
• Grantee: Camila Infantosi Vannucchi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/24731-2 - Oxidative stress and antioxidant profile evaluation in the neonatal period of puppies born by vaginal eutocia or elective cesarean section
• Grantee: Leticia Lima de Almeida
• Support Opportunities: Scholarships in Brazil - Master