Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sphingolipids signature in plasma and tissue as diagnostic and prognostic tools in oral squamous cell carcinoma

Full text
Author(s):
Faedo, Raquel Roman [1] ; da Silva, Gabriel [1] ; da Silva, Rodrigo Moreira [2] ; Ushida, Tatiane Resende [1] ; da Silva, Ricardo Roberto [2] ; Lacchini, Riccardo [3] ; Matos, Leandro Luongo [4] ; Kowalski, Luiz Paulo [4] ; Lopes, Noberto Peporine [2] ; Leopoldino, Andreia Machado [1, 5]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Av Cafe S-N, Bloco A, Sala 99A, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Nucleo Pesquisa Prod Nat & Sintet NPPNS, Dept Ciencias Biomol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Coll Nursing, Dept Psychiat Nursing & Human Sci, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Fac Med LIM28, Hosp Clin HCFMUSP, Head & Neck Surg Dept, Inst Canc Estado Sao Paulo, Sao Paulo - Brazil
[5] Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Therapy, CEPID FAPESP, Ribeirao Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS; v. 1867, n. 1 JAN 2022.
Web of Science Citations: 0
Abstract

Enzymes related to sphingolipids metabolism has been suggested as altered in oral squamous cell carcinoma (OSCC). However, clinical relevance of diverse sphingolipids in OSCC is not fully known. Here, we evaluated sphingolipidomics in plasma and tumor tissues as a tool for diagnosis/prognosis in OSCC patients. Plasma was obtained from 58 controls and 56 OSCC patients, and paired tumor and surgical margin tissues (n = 42). The levels of 28 sphingolipids molecules were obtained by mass spectrometry. Furthermore, sphingolipids were analyzed with clinical and pathological characteristics to search the potential for diagnosis and prognosis. Lower levels of 17 sphingolipids was found in the plasma of OSCC patients compared to controls while four were elevated in tumor tissues. C18:0 dyhidroceramide and C24:0 lactosylceramide in plasma were associated with perineural invasion, while tissue levels of ceramide and dyhidroceramide were associated with advanced tumor stage and perineural invasion. High plasma levels of C24:0 ceramide (HR = 0.10, p = 0.0036) and C24:1 glucosylceramide (HR = 6.62, p = 0.0023), and tissue levels of C24:0 dyhidroceramide (HR = 3.95, p = 0.032) were identified as independent prognostic factors. Moreover, we identified signatures composed by i) sphinganine-1-phosphate and C16 ceramide-1-phosphate in plasma with significant diagnostic accuracy, while ii) C24:0 ceramide, C24:0 dyhidroceramide, and C24:1 glucosylceramide plasma levels, and iii) C24:0 dyhidrosphingomyelin and C24:0 ceramide tissue levels showed value to predict survival in patients aged 60 years or older. We proposed the sphingolipids signatures in plasma and tumor tissues as biomarkers candidates to diagnosis and prognosis in OSCC. (AU)

FAPESP's process: 17/18922-2 - Development of a computing platform extensible and modular for metabolomics and metagenomics analysis: innovation with the discovery of new enzymatic activities and natural products of pharmaceutical interest derived
Grantee:Ricardo Roberto da Silva
Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants
FAPESP's process: 18/17480-9 - Study of SET and sphingolipids in head and neck cancer: signaling, targets and antitumor therapy
Grantee:Gabriel da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/19103-2 - SET and sphingolipids in head and neck squamous cell carcinoma: signaling, targets and antiumoral therapy
Grantee:Andréia Machado Leopoldino
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/05026-4 - Development of a computational platform extensible and modular for analysis of metabolomics and metagenomics experiments: Innovating with the discovery of new enzymatic activities and derived natural products of interest
Grantee:Ricardo Roberto da Silva
Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers
FAPESP's process: 18/03278-3 - Profile of sphingolipids in head and neck cancer
Grantee:Raquel Roman Faedo
Support Opportunities: Scholarships in Brazil - Master