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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

species dilemma: utilizing biological species concept to reconcile DNA barcode and morphological divergences for Hypnea pseudomusciformis (Gigartinales, Rhodophyta

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Author(s):
Nauer, Fabio [1] ; Marchi, Fabiana [1] ; Naves, Matheus [1] ; Plastino, Estela Maria [1] ; Oliveira, Mariana Cabral [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Biosci, Dept Bot, Rua Matao 277, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF APPLIED PHYCOLOGY; v. 34, n. 1 NOV 2021.
Web of Science Citations: 0
Abstract

Taxonomic studies based on molecular markers led to the description of a new species, Hypnea pseudomusciformis, to accommodate three morphologically divergent species that occur on the coast of Brazil and have identical DNA barcode sequences (H. musciformis, H. nigrescens, and Hypnea valentiae). These three previous species were then identified as morphological variants of H. pseudomusciformis. Later, phylogeographic studies revealed that H. pseudomusciformis is subdivided in three distinct haplotype groups with significant intraspecific divergence for the COI-5P marker. Moreover, each haplogroup presents physiological and morphological differences related to each of the three floristic provinces along the Brazilian coast: tropical, transition, and warm temperate. Therefore, these haplogroups were denominated as ecotypes. In this work, we tested the biological species concept (BSC) in two crossing experiments: (i) between specimens belonging to different haplogroups (obtained from tropical and warm temperate provinces) and (ii) between different morphological variants ({''}musciformis{''} and ``nigrescens{''}) belonging to the same haplogroup. As a result, we observed that all crosses performed were positive and the reach of fertility in all tetrasporophytic plants of the F1 and F2 generations. Therefore, we concluded that (i) specimens from tropical and warm temperate provinces and (ii) specimens identified as ``musciformis{''} and ``nigrescens{''} belong to the same biological entity, despite their intraspecific molecular divergences and physiological differences and their differences in morphology, respectively. When testing and confirming the biological concept of species, we also validate previous works or confirm that the species has great phenotypic and physiological plasticity, which allows its occurrence in different niches and in provinces with different abiotic characteristics. The BSC proved to be a valuable tool for responding to conflicts raised by the DNA barcode technique. (AU)

FAPESP's process: 19/08734-0 - Effect of temperature and nutrients on color variants of Hypnea pseudomusciformis (Rhodophyta) and verification of genetic pattern for color inheritance
Grantee:Matheus Naves de Almeida
Support Opportunities: Scholarships in Brazil - Scientific Initiation