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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter

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Author(s):
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Mechler-Dreibi, Marina L. [1] ; Almeida, Henrique M. S. [1] ; Sonalio, Karina [1] ; Martines, Mariela A. C. [1] ; Petri, Fernando A. M. [1] ; Zambotti, Beatriz B. [1] ; Ferreira, Marcela M. [1] ; Storino, Gabriel Y. [1] ; Martins, Tereza S. [2] ; Montassier, Helio J. [1] ; Sant'Anna, Osvaldo A. [3] ; Fantini, Marcia C. A. [4] ; de Oliveira, Luis Guilherme [1]
Total Authors: 13
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci, Jaboticabal, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Dept Chem, Diadema, SP - Brazil
[3] Butantan Inst, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 11, n. 1 NOV 17 2021.
Web of Science Citations: 0
Abstract

Mycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection. (AU)

FAPESP's process: 18/12742-5 - Oral immunization for increased immune response in the control of Mycoplasma hyopneumoniae infection in swine
Grantee:Marina Lopes Mechler Dreibi
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/17844-8 - Nanostructured silica as a protective vehicle for vaccines and biomolecules
Grantee:Osvaldo Augusto Brazil Esteves Sant'Anna
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/19710-4 - Mycoplasma hyopneumoniae infection: pathogenic factors, mechanisms of response and alternative strategies of immunological stimulation
Grantee:Luís Guilherme de Oliveira
Support Opportunities: Regular Research Grants