Genomic Analysis of Carbapenem-Resistant Pseudomonas aeruginosa Isolated From Urban Rivers Confirms Spread of Clone Sequence Type 277 Carrying Broad Resistome and Virulome Beyond the Hospital

Author(s)

Esposito, Fernanda ^{[1, 2]} ; Cardoso, Brenda ^{[3, 2]} ; Fontana, Herrison ^{[1, 2]} ; Fuga, Bruna ^{[3, 1, 2]} ; Cardenas-Arias, Adriana ^{[3, 2]} ; Moura, Quezia ^[4] ; Fuentes-Castillo, Danny ^{[5, 2]} ; Lincopan, Nilton ^{[3, 1, 2]}

Affiliation

^[1] Univ Sao Paulo, Sch Pharm, Dept Clin Anal, Sao Paulo - Brazil

^[2] One Hlth Brazilian Resistance Project OneBR, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[4] Fed Inst Educ Sci & Technol Espirito Santo, Vila Velha - Brazil

^[5] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: FRONTIERS IN MICROBIOLOGY; v. 12, SEP 3 2021.
• Web of Science Citations: 0

Abstract

The dissemination of antibiotic-resistant priority pathogens beyond hospital settings is both a public health and an environmental problem. In this regard, high-risk clones exhibiting a multidrug-resistant (MDR) or extensively drug-resistant (XDR) phenotype have shown rapid adaptation at the human-animal-environment interface. In this study, we report genomic data and the virulence potential of the carbapenemase, Sao Paulo metallo-beta-lactamase (SPM-1)-producing Pseudomonas aeruginosa strains (Pa19 and Pa151) isolated from polluted urban rivers, in Brazil. Bioinformatic analysis revealed a wide resistome to clinically relevant antibiotics (carbapenems, aminoglycosides, fosfomycin, sulfonamides, phenicols, and fluoroquinolones), biocides (quaternary ammonium compounds) and heavy metals (copper), whereas the presence of exotoxin A, alginate, quorum sensing, types II, III, and IV secretion systems, colicin, and pyocin encoding virulence genes was associated with a highly virulent behavior in the Galleria mellonella infection model. These results confirm the spread of healthcare-associated critical-priority P. aeruginosa belonging to the MDR sequence type 277 (ST277) clone beyond the hospital, highlighting that the presence of these pathogens in environmental water samples can have clinical implications for humans and other animals. (AU)

• FAPESP's process: 20/08224-9 - OneBR (One Health Brazilian Resistance): integrated genomic database for surveillance, diagnosis, management and treatment of antimicrobial resistance in the human-animal-environment interface, in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/15578-4 - Virulome and pathogenicity of carbapenem- and polymyxin-resistant priority pathogens
• Grantee: Fernanda Ribeiro dos Santos Esposito
• Support Opportunities: Scholarships in Brazil - Doctorate