Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Wide visible-range activatable fluorescence ZnSe:Eu3+/Mn2+@ZnS quantum dots: local atomic structure order and application as a nanoprobe for bioimaging

Full text
Author(s):
Khan, Zahid Ullah [1, 2] ; Uchiyama, Mayara Klimuk [1] ; Khan, Latif Ullah [1, 3] ; Araki, Koiti [1] ; Goto, Hiro [4] ; Franca Cunha Felinto, Maria Claudia [5] ; de Souza, Ana Olivia [6] ; de Brito, Hermi Felinto [1] ; Gidlund, Magnus [2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci 4, Dept Immunol, BR-05508000 Sao Paulo, SP - Brazil
[3] Synchrotron Light Expt Sci & Applicat Middle East, POB 7, Allan 19252 - Jordan
[4] Univ Sao Paulo, Fac Med, BR-05403000 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Inst Nucl Energy & Res, BR-05508000 Sao Paulo, SP - Brazil
[6] Butantan Inst, Dev & Innovat Lab, BR-05503900 Sao Paulo, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: JOURNAL OF MATERIALS CHEMISTRY B; DEC 2021.
Web of Science Citations: 0
Abstract

The development of QDs-based fluorescent bionanoprobe for cellular imaging fundamentally relies upon the precise knowledge of particle-cell interaction, optical properties of QDs inside and outside of the cell, movement of a particle in and out of the cell, and the fate of particle. We reported engineering and physicochemical characterization of water-dispersible Eu3+/Mn2+ co-doped ZnSe@ZnS core/shell QDs and studied their potential as a bionanoprobe for biomedical applications, evaluating their biocompatibility, fluorescence behaviour by CytoViva dual mode fluorescence imaging, time-dependent uptake, endocytosis and exocytosis in RAW 264.7 macrophages. The oxidation state and local atomic structure of the Eu dopant studied by X-ray absorption fine structure (XAFS) analysis manifested that the Eu3+ ions occupied sites in both ZnSe and ZnS lattices for the core/shell QDs. A novel approach was developed to relieve the excitation constraint of wide bandgap ZnSe by co-incorporation of Eu3+/Mn2+ codopants, enabling the QDs to be excited at a wide UV-visible range. The QDs displayed tunable emission colors by a gradual increase in Eu3+ concentration at a fixed amount of Mn2+, systematically enhancing the Mn2+ emission intensity via energy transfer from the Eu3+ to Mn2+ ion. The ZnSe:Eu3+/Mn2+@ZnS QDs presented high cell viability above 85% and induced no cell activation. The detailed analyses of QDs-treated cells by dual mode fluorescence CytoViva microscopy confirmed the systematic color-tunable fluorescence and its intensity enhances as a function of incubation time. The QDs were internalized by the cells predominantly via macropinocytosis and other lipid raft-mediated endocytic pathways, retaining an efficient amount for 24 h. The unique color tunability and consistent high intensity emission make these QDs useful for developing a multiplex fluorescent bionanoprobe, activatable in wide-visible region. (AU)

FAPESP's process: 21/00356-6 - Multifunctional fluorescence quantum dots as amplifiers for 1O2 generation and synergistic enhanced photodynamic therapy
Grantee:Zahid Ullah Khan
Support Opportunities: Scholarships in Brazil - Post-Doctoral