Low Mg content on Ti-Nb-Sn alloy when in contact w... - BV FAPESP
Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions

Full text
Author(s):
Dias, Carolina da Silva [1] ; Rossi, Mariana Correa [1] ; Apolonio, Emanuel V. P. [1] ; Rosa, Gustavo dos Santos [1] ; Hubbe Pfeifer, Joao Pedro [1] ; Hussni, Carlos Alberto [1] ; Watanabe, Marcos Jun [1] ; Alves, Ana Liz Garcia [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, Dept Vet Surg & Anesthesiol, Julio de Mesquita Filho Unesp Prof Doutor Walter, BR-18618681 Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 32, n. 12 DEC 2021.
Web of Science Citations: 0
Abstract

Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse (R) Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release. (AU)

FAPESP's process: 19/09752-1 - Evaluation of the potential of osteogenic differentiation and oxidative stress of mesenchymal stem cells derived from bone marrow in contact with porous titanium alloy
Grantee:Carolina da Silva Dias
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/14460-4 - EFFECT OF INTRAARTICULAR INJECTION OF MICROCAPSULES WITH MESENCHYMAL STEM CELLS ON THE TREATMENT OF OSTEOARTHRITIS
Grantee:Ana Liz Garcia Alves
Support Opportunities: Regular Research Grants
FAPESP's process: 17/13876-2 - Characterization of Ti-Nb-Sn alloy with Mg and its Biotechnological Potential Bone Fracture Repair.
Grantee:Mariana Correa Rossi
Support Opportunities: Scholarships in Brazil - Doctorate