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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis

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Author(s):
Girol, Ana Paula [1, 2, 3] ; Zanon, Caroline de Freitas [2] ; Caruso, Icaro Putinhon [4] ; Costa, Sara de Souza [1, 2] ; Souza, Helena Ribeiro [1, 2] ; Cornelio, Marinonio Lopes [4] ; Oliani, Sonia Maria [2, 3, 5]
Total Authors: 7
Affiliation:
[1] Univ Ctr Padre Albino UNIFIPA, Dept Phys & Morphol Sci, BR-15809144 Catanduva, SP - Brazil
[2] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci Ibilce, Dept Biol, UNESP, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med UNIFESP EPM, Post Grad Program Struct & Funct Biol, BR-04023062 Sao Paulo, SP - Brazil
[4] Sao Paulo State Univ, Inst Biosci, Dept Phis, UNESP, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[5] Uniao Fac Grandes Lagos Unilago, Adv Res Ctr Med CEPAM, BR-15030070 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: CELLS; v. 10, n. 11 NOV 2021.
Web of Science Citations: 0
Abstract

Uveitis is one of the main causes of blindness worldwide, and therapeutic alternatives are worthy of study. We investigated the effects of piperlongumine (PL) and/or annexin A1 (AnxA1) mimetic peptide Ac2-26 on endotoxin-induced uveitis (EIU). Rats were inoculated with lipopolysaccharide (LPS) and intraperitoneally treated with Ac2-26 (200 mu g), PL (200 and 400 mu g), or Ac2-26 + PL after 15 min. Then, 24 h after LPS inoculation, leukocytes in aqueous humor, mononuclear cells, AnxA1, formyl peptide receptor (fpr)1, fpr2, and cyclooxygenase (COX)-2 were evaluated in the ocular tissues, along with inflammatory mediators in the blood and macerated supernatant. Decreased leukocyte influx, levels of inflammatory mediators, and COX-2 expression confirmed the anti-inflammatory actions of the peptide and pointed to the protective effects of PL at higher dosage. However, when PL and Ac2-26 were administered in combination, the inflammatory potential was lost. AnxA1 expression was elevated among groups treated with PL or Ac2-26 + PL but reduced after treatment with Ac2-26. Fpr2 expression was increased only in untreated EIU and Ac2-26 groups. The interaction between Ac2-26 and PL negatively affected the anti-inflammatory action of Ac2-26 or PL. We emphasize that the anti-inflammatory effects of PL can be used as a therapeutic strategy to protect against uveitis. (AU)

FAPESP's process: 15/03359-5 - Endotoxin-induced uveitis: analysis of the interaction Annexin A1 and natural bioactive extracts as an alternative therapy
Grantee:Ana Paula Girol
Support type: Regular Research Grants
FAPESP's process: 19/19949-7 - Evaluation of annexin A1 protein immunomodulatory activity in cutaneous and placental lesions resulting from Diabetes mellitus
Grantee:Sonia Maria Oliani
Support type: Regular Research Grants