Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis

Full text
Girol, Ana Paula [1, 2, 3] ; Zanon, Caroline de Freitas [2] ; Caruso, Icaro Putinhon [4] ; Costa, Sara de Souza [1, 2] ; Souza, Helena Ribeiro [1, 2] ; Cornelio, Marinonio Lopes [4] ; Oliani, Sonia Maria [2, 3, 5]
Total Authors: 7
[1] Univ Ctr Padre Albino UNIFIPA, Dept Phys & Morphol Sci, BR-15809144 Catanduva, SP - Brazil
[2] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci Ibilce, Dept Biol, UNESP, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med UNIFESP EPM, Post Grad Program Struct & Funct Biol, BR-04023062 Sao Paulo, SP - Brazil
[4] Sao Paulo State Univ, Inst Biosci, Dept Phis, UNESP, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[5] Uniao Fac Grandes Lagos Unilago, Adv Res Ctr Med CEPAM, BR-15030070 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: CELLS; v. 10, n. 11 NOV 2021.
Web of Science Citations: 0

Uveitis is one of the main causes of blindness worldwide, and therapeutic alternatives are worthy of study. We investigated the effects of piperlongumine (PL) and/or annexin A1 (AnxA1) mimetic peptide Ac2-26 on endotoxin-induced uveitis (EIU). Rats were inoculated with lipopolysaccharide (LPS) and intraperitoneally treated with Ac2-26 (200 mu g), PL (200 and 400 mu g), or Ac2-26 + PL after 15 min. Then, 24 h after LPS inoculation, leukocytes in aqueous humor, mononuclear cells, AnxA1, formyl peptide receptor (fpr)1, fpr2, and cyclooxygenase (COX)-2 were evaluated in the ocular tissues, along with inflammatory mediators in the blood and macerated supernatant. Decreased leukocyte influx, levels of inflammatory mediators, and COX-2 expression confirmed the anti-inflammatory actions of the peptide and pointed to the protective effects of PL at higher dosage. However, when PL and Ac2-26 were administered in combination, the inflammatory potential was lost. AnxA1 expression was elevated among groups treated with PL or Ac2-26 + PL but reduced after treatment with Ac2-26. Fpr2 expression was increased only in untreated EIU and Ac2-26 groups. The interaction between Ac2-26 and PL negatively affected the anti-inflammatory action of Ac2-26 or PL. We emphasize that the anti-inflammatory effects of PL can be used as a therapeutic strategy to protect against uveitis. (AU)

FAPESP's process: 15/03359-5 - Endotoxin-induced uveitis: analysis of the interaction Annexin A1 and natural bioactive extracts as an alternative therapy
Grantee:Ana Paula Girol
Support type: Regular Research Grants
FAPESP's process: 19/19949-7 - Evaluation of annexin A1 protein immunomodulatory activity in cutaneous and placental lesions resulting from Diabetes mellitus
Grantee:Sonia Maria Oliani
Support type: Regular Research Grants