| Full text | |
| Author(s): |
Bendia, Amanda G.
[1]
;
Callefo, Flavia
[2]
;
Araujo, Maicon N.
[3]
;
Sanchez, Evelyn
[4]
;
Teixeira, Veronica C.
[2]
;
Vasconcelos, Alessandra
[4]
;
Battilani, Gislaine
[4]
;
Pellizari, Vivian H.
[1]
;
Rodrigues, Fabio
[3]
;
Galante, Douglas
[2]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Oceanog Inst, Biol Oceanog Dept, Praca Oceanog 191, BR-05508120 Sao Paulo - Brazil
[2] Brazilian Ctr Res Energy & Mat, Brazilian Synchrotron Light Lab, Campinas - Brazil
[3] Univ Sao Paulo, Fundamental Chem Dept, Inst Chem, Sao Paulo - Brazil
[4] Fed Univ Jequitinhonha & Mucuri, Inst Sci & Technol, Diamantina - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | ASTROBIOLOGY; OCT 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Microbial communities have been explored in various terrestrial subsurface ecosystems, showing metabolic potentials that could generate noteworthy morphological and molecular biosignatures. Recent advancements in bioinformatic tools have allowed for descriptions of novel and yet-to-be cultivated microbial lineages in different ecosystems due to the genome reconstruction approach from metagenomic data. Using shotgun metagenomic data, we obtained metagenome-assembled genomes related to cultivated and yet-to-be cultivated prokaryotic lineages from a silica and iron-rich cave (Monte Cristo) in Minas Gerais State, Brazil. The Monte Cristo Cave has been shown to possess a high diversity of genes involved with different biogeochemical cycles, including reductive and oxidative pathways related to carbon, sulfur, nitrogen, and iron. Three genomes were selected for pangenomic analysis, assigned as Truepera sp., Ca. Methylomirabilis sp., and Ca. Koribacter sp. based on their lifestyles (radiation resistance, anaerobic methane oxidation, and potential iron oxidation). These bacteria exhibit genes involved with multiple DNA repair strategies, starvation, and stress response. Because these groups have few reference genomes deposited in databases, our study adds important genomic information about these lineages. The combination of techniques applied in this study allowed us to unveil the potential relationships between microbial genomes and their ecological processes with the cave mineralogy and highlight the lineages involved with anaerobic methane oxidation, iron oxidation, and radiation resistance as functional models for the search for extant life-forms outside our planet in silica- and iron-rich environments and potentially on Mars. (AU) | |
| FAPESP's process: | 21/05083-8 - Nanoscale multitechnical synchrotron characterization applied to the problem of biogenicity of minerals |
| Grantee: | Douglas Galante |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/23241-0 - Metagenomic of microbial communities from environments with geothermal activity at Deception Island Volcano, Antarctica |
| Grantee: | Amanda Gonçalves Bendia |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/06114-6 - The Neoproterozoic Earth System and the rise of biological complexity |
| Grantee: | Ricardo Ivan Ferreira da Trindade |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 20/02537-5 - Exploring Nanopaleontology: new parameters of biogenicity and the study of biosignatures with synchrotron light |
| Grantee: | Flávia Callefo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |