| Full text | |
| Author(s): |
Andrade Brito, Eric Saymom
[1]
;
Taborda Prado, Lais Di Paulie
[1]
;
Costa Araujo, Liana Kesia
[1]
;
Arnhold, Emmanuel
[2]
;
Chediak Matos, Moema Pacheco
[1]
;
Marciano de Paula, Joelma Abadia
[3]
;
Ramos, Luciana Machado
[3]
;
Fonseca-Alves, Carlos Eduardo
[4, 5]
;
Brianezi Dignani de Moura, Veridiana Maria
[1]
Total Authors: 9
|
| Affiliation: | [1] Fed Univ Goias UFG, Anim Pathol Serv, Goiania, Go - Brazil
[2] Fed Univ Goias UFG, Dept Anim Sci, Goiania, Go - Brazil
[3] Goias State Univ, Unit Exact & Technol Sci, Anapolis, Go - Brazil
[4] Sao Paulo State Univ UNESP, Dept Vet Surg & Anim Reprod, Botucatu, SP - Brazil
[5] Univ Sao Paulo UNIP, Inst Hlth Sci, Bauru, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | FRONTIERS IN VETERINARY SCIENCE; v. 8, APR 12 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Prostatic cancer (PC) stands out in terms of its occurrence, pathophysiology, and unfavorable prognostics in humans and dogs. Natural drugs bear an integrative potential for conventional antineoplastic treatments. In this context, the bioproducts of Synadenium grantii have been empirically used in different parts of Brazil for the integrative treatment of prostate cancer in humans. However, there is no availability of scientific evidence of the antitumor effects of S. grantii. Therefore, this study aimed to investigate the bioactive compounds in the latex of S. grantii using the high-resolution mass spectrophotometry (HRMS) and to evaluate its cytotoxic effects on primary canine PC cell cultures. Four fragments of phorbol ester were identified as potential bioactive compounds using the HRMS. With the help of an MTT ({[}3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide]) assay, two canine prostatic carcinoma cell lines (PC 1 and PC2) showed a decrease in the tumor cell count, with an Inhibitory concentration 50 (IC50)of 0.8469 and 0.6068 mg/ml, respectively, for PC1 and PC2. In conclusion, the latex of S. grantii contains phorbol esters in its composition, and its aqueous solution has a cytotoxic effect on canine metastatic PC cells in vitro. (AU) | |
| FAPESP's process: | 19/24079-1 - Antitumor effect of Synadenium grantii Hook F. latex on preclinical models of mammary gland tumors |
| Grantee: | Carlos Eduardo Fonseca Alves |
| Support Opportunities: | Regular Research Grants |