| Full text | |
| Author(s): |
Tsuchiya, Ana Claudia
[1]
;
Gomes, Erika Santos
[2]
;
Kuaye, Arnaldo Yoshiteru
[1]
;
Kabuki, Dirce Yorika
[2]
Total Authors: 4
|
| Affiliation: | [1] Univ Estadual Campinas, Dept Food Technol, Campinas - Brazil
[2] Univ Estadual Campinas, Food Sci Dept, Campinas - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL; v. 28, n. 1, p. 85-92, JAN 2022. |
| Web of Science Citations: | 0 |
| Abstract | |
The aims of this study were to evaluate the occurrence of Clostridium difficile in commercial raw meat and meat products commercialized in Brazil, and to determine the pathogenic potential and antimicrobial susceptibility of the isolates. After selective enrichment, the isolation of C. difficile involved plating with and without an alcohol shock treatment onto C. difficile moxalactam agar (CDMNA). The toxigenic profile was determined through PCR for detection of tcdA, tcdB, cdtA and cdtB genes and an enzyme-linked immunosorbent assay for toxin A/B. C. difficile was isolated from 8.9% (17 out of 192) of analyzed samples. Plating without alcohol treatment (sensitivity of 88.23%) was more efficient than with alcohol treatment (sensitivity of 29.41%) in C. difficile isolation. The profile A + B+CDT- was observed in 35.0% (28/80) of the isolates. The A/B toxin was tested in 44 isolates and 15.9% of them were positive. Resistance to clindamycin, ceftizoxime tetracycline, metronidazole, vancomycin, and ceftriaxone were observed among isolates. Multi-drug resistance was detected in 36.4% (8/22) of the isolates evaluated. (AU) | |
| FAPESP's process: | 09/52707-5 - Evaluation of Clostridium difficile as emergent anaerobic pathogen in meats |
| Grantee: | Dirce Yorika Kabuki |
| Support Opportunities: | Regular Research Grants |