ariant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus

Author(s):Show less -

Herkenhoff, Marcos Edgar ^[1] ; Bovolenta, Luiz A. ^[2] ; Broedel, Oliver ^[3] ; dos Santos, Lucilene D. ^[4] ; de Oliveira, Arthur C. ^[1] ; Chuffa, Luiz G. A. ^[5] ; Ribeiro, Amanda de O. ^[1] ; Lupi, Luiz A. ^[5] ; Dias, Marco A. D. ^{[6, 7]} ; Hilsdorf, Alexandre W. S. ^{[6, 7]} ; Frohme, Marcus ^[3] ; Pinhal, Danillo ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Chem & Biol Sci, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Biophys & Pharmacol, Botucatu, SP - Brazil

^[3] Tech Univ Appl Sci Wildau, Div Mol Biotechnol & Funct Genom, Wildau - Germany

^[4] Sao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Struct & Funct Biol, Botucatu, SP - Brazil

^[6] Univ Fed Lavras, Dept Anim Sci, Lavras, MG - Brazil

^[7] Univ Mogi das Cruzes, Unit Biotechnol, Mogi Das Cruzes, SP - Brazil

• Document type: Journal article
• Source: Genomics; v. 113, n. 6, p. 4303-4312, NOV 2021.
• Web of Science Citations: 0

Abstract

Nile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOFMS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed. (AU)

• FAPESP's process: 14/03062-0 - Molecular characterization of heterotic hybrid from Red Stirling and Chitralada lines of Nile Tilapia (Oreochromis niloticus)
• Grantee: Marcos Edgar Herkenhoff
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/23752-2 - Quantitative and molecular evaluation of a continuous crossbreeding system using Oreochromis niloticus Chitralada and red stirling strains
• Grantee: Alexandre Wagner Silva Hilsdorf
• Support Opportunities: Regular Research Grants