Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Methacrylation of epigallocatechin-gallate for covalent attachment with a dental polymer

Full text
Author(s):
Bortolatto, Janaina Freitas [1] ; Buzalaf, Marilia Rabelo Afonso [1] ; Ebrahimi, Jessica [2] ; Floros, Michael Christopher [3] ; Ho, Mayhay [3] ; Prakki, Anuradha [3]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Sao Paulo - Brazil
[2] Univ Toronto, Fac Dent, Dept Clin Sci Orthodont Discipline, Toronto, ON M5G 1G6 - Canada
[3] Univ Toronto, Fac Dent, Dent Res Inst Restorat Discipline, Toronto, ON M5G 1G6 - Canada
Total Affiliations: 3
Document type: Journal article
Source: Dental Materials; v. 37, n. 12, p. 1751-1760, DEC 2021.
Web of Science Citations: 0
Abstract

Objective. Synthesize novel epigallocatechin-gallate (EGCG) methacrylate monomers with the ability to copolymerize with dental methacrylate resins. Methods. EGCG was reacted with 1/3 (E33), 2/3 (E67) and 1 (E100) molar equivalents of methacyloyl chloride introducing three degrees of polymerizablility. EGCG-methacrylates were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR). E33, E67, E100 and neat EGCG were incorporated into TEGDMA at 0.5-20% ratios (m/m). Copolymers were tested for degree of conversion (%DC), EGCG release, gel content (%GC), degree of swelling (%DS), flexural properties and bacterial viability (Streptococcus mutans, baseline/30-days). Neat TEGDMA and TEGDMA passively loaded with EGCG (E0) were used as controls. Data were analysed by one-way ANOVA, Tukey, and Dunnett's method ( = 5%). Two-way ANOVA and Bonferroni were used to investigate factor interaction. Results. FTIR/NMR confirmed synthesis of desired compounds. All of E100 incorporated ratios had %DC similar to TEGDMA. Remaining groups had reduction in %DC at 2% in E0, 10% in E33 and 20% in E67 ratios. EGCG was stable within ECGC-methacrylate copolymers. Release of EGCG from E0 significantly increased with higher EGCG ratios. Except for E100, higher EGCG or EGCG-methacrylate ratios led to decreased %CG and %DS. At baseline, E0 had the lowest bacterial survival rates (1-10% survival) at all ratios compared to E33, E67, E100, and neat TEGDMA. However, E33, E67 and E100 still had statistically lower survival rates (7-53%) compared with neat TEGDMA. After 30-days, all compounds had similar survival rates for all ratios, which were lower than that of neat TEGDMA. Significance. Demonstration of methacrylate functionalized EGCG- with inherited antibacterial activity for improved restoration longevity. (c) 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 17/09827-6 - Novel bioinspired bonding agents for improving longevity of dental restorations
Grantee:Janaína Freitas Bortolatto
Support Opportunities: Scholarships in Brazil - Post-Doctoral