rotein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluatio

Author(s):Show less -

Araujo, Tamara Teodoro ^[1] ; Carvalho, Thamyris Souza ^[1] ; Dionizio, Aline ^[1] ; Bogaz Debortolli, Ana Luiza ^[1] ; Oliveira Ventura, Talita Mendes ^[1] ; Souza, Beatriz Martines ^[1] ; Vilas Boas Feitosa, Chelsea Maria ^[1] ; Pereira Barbosa, Heloisa Aparecida ^[1] ; da Silva Ribeiro, Caroline Fernanda ^[1] ; Martini, Tatiana ^[1] ; Taira, Even ^[1] ; Tanaka Shibao, Priscila Yumi ^[2] ; Henrique-Silva, Flavio ^[2] ; Marchetto, Reinaldo ^[3] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Technol, Araraquara, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 116, JAN 2022.
• Web of Science Citations: 0

Abstract

Objective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination. Materials and methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H2O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88x10(-)5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2-2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7-25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined. Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP. (AU)

• FAPESP's process: 19/19002-0 - Proteomic analysis of acquired pellicle dental enamel: effect of cystatin, staterin and hemoglobin against dental erosion
• Grantee: Ana Luiza Bogaz Debortolli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/08032-5 - Acquired pellicle engineering for protection against caries and tooth erosion: effect of cystatin, statherin and hemoglobin on the proteomic profile of the acquired pellicle, bacterial biofilm and dental enamel
• Grantee: Tamara Teodoro Araujo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Research Projects - Thematic Grants