Isopropanol-butanol-ethanol production by cell-immobilized vacuum fermentation

Author(s)

dos Santos Vieira, Carla Ferreira ^[1] ; Sia, Augusto Duzi ^[1] ; Maugeri Filho, Francisco ^[2] ; Maciel Filho, Rubens ^[1] ; Mariano, Adriano Pinto ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Lab Optimizat Design & Adv Control Fermentat Div, Sch Chem Engn, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Food Engn, Bioproc & Metab Engn Lab LEMeB, Campinas, SP - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 344, n. B JAN 2022.
• Web of Science Citations: 0

Abstract

The Isopropanol-Butanol-Ethanol productivity by solventogenic clostridia can increase when cells are immobilized on low-cost, renewable fibrous materials; however, butanol inhibition imposes the need for dilute sugar solutions (less than40 g/L). To alleviate this problem, the in-situ vacuum product recovery technique was applied to recover IBE in repeated-batch cultivation of Clostridium beijerinckii DSM 6423 immobilized on sugarcane bagasse. Five repeated batch cycles were conducted in a 7-L bioreactor containing P2 medium (-60 g/L glucose) and bagasse packed in 3D-printed concentric annular baskets. In three cycles, glucose was consumed by 86% on average, the IBE productivity was 0.35 g/L center dot h or 30% and 17% higher relative to free-and immobilized (without vacuum)-cell cultures. Notably, the product stream contained 45 g/L IBE. However, the fermentation was unsatisfactory in two cycles. Finally, by inserting a fibrous bed with hollow annuli in a vacuum fermentation, this work introduces the concept of an internal-loop boiling-driven fibrous-bed bioreactor. (AU)

• FAPESP's process: 18/23983-3 - Routes for production of sugarcane-based tires: efficient conversion of sugars to 1,3-butadiene precursors and techno-economic-environmental assessment
• Grantee: Adriano Pinto Mariano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/23042-9 - Development of advanced fermentation technology for the production of n-butanol, isopropanol and ethanol from sugar cane bagasse
• Grantee: Carla Ferreira dos Santos Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate