Comparison of two volumes for sciatic perineural injection using 1% methylene blue in rabbit cadavers

Author(s)

Jane, D. R. ^[1] ; Gusmao, B. S. ^[1] ; Oliveira, B. B. ^[1] ; Custodio, L. P. ^[1] ; Reis Filho, N. P. ^[1] ; Trein, T. A. ; Floriano, B. P. ^[1]

Affiliation

^[1] Ctr Univ Fac Integradas Ourinhos, Ourinhos, SP - Brazil

• Document type: Journal article
• Source: Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 73, n. 6, p. 1309-1314, NOV-DEC 2021.
• Web of Science Citations: 0

Abstract

ABSTRACT The purpose of this study was to assess the efficacy of a blind technique for sciatic and femoral nerve block in rabbit cadavers by evaluating the spread of 1% methylene blue at two different volumes. Nine recently euthanized rabbits weighing 2.5(0.3kg were used. The sciatic (SN) and femoral (FN) nerves of each limb were randomly assigned for injection with 1% methylene blue at 0.2mL/kg (G0.2) or 0.3mL/kg (G0.3). Nerves were dissected and measured for depth and extension of staining (cm). Mean comparisons were performed using paired t test. The relation between volume and nerve staining ( 2cm was assessed using chi-square test. The mean depth of SN was 1.9±0.2 and 1.6±0.3cm and staining 1.9±1.4 and 2.0±1.2cm, respectively in G0.2 and G0.3. No relation was found between depth and dye spread and there was no association between nerve staining ( 2.0cm and volume of solution. The FN failed to be stained in all subjects. In conclusion, SN injection can be successfully performed without guidance in rabbits. The lower volume (0.2mL/kg) is recommended to avoid systemic toxicity. (AU)

• FAPESP's process: 17/17880-4 - Sciatic and femoral nerve block in cats: anatomical study and determination of an apropriate volume for blind injection
• Grantee: Daniela Ribas Jané
• Support Opportunities: Scholarships in Brazil - Scientific Initiation