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Effects of perineural administration of dexmedetomidine combined with ropivacaine 0.75% in sciatic and femoral nerve blocks guided by ultrasound and electrical nerve stimulation in dogs

Grant number: 14/10449-8
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2014
Effective date (End): July 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Valéria Nobre Leal de Souza Oliva
Grantee:Thomas Alexander Trein
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The interest in regional anesthetic block in companion animals has increased significantly over the last years. The femoral and sciatic nerve blocks are an alternative to neuraxial anesthesia for pelvic limbs and present similar analgesic and anesthetic effects; however, with lower incidence of complications. The combination of adjuvants with local anesthetics is currently being studied with the aim to increase quality and safety of the peripheral anesthetic block. This study aims to investigate the effects of dexmedetomidine, administered either perineurally or systemically, combined with femoral and sciatic nerve blocks with ropivacaine 0.75% in dogs. More specifically, this study will evaluate and compare onset times and duration of motor and sensory blockade, as well as degree of anesthetic blockade obtained by these three protocols. Moreover, this study will evaluate the potential side effects of dexmedetomidine, administered perineurally or systemically, upon cardiorespiratory parameters, as well as complications related to the technique. Seven healthy Beagle dogs, males and females, aged two to four years and weighing five to 15 kilos will be used. Each dog will participate in three experimental groups: perineural administration of ropivacaine 0.75% on the aforementioned nerves (0.1mL/kg/nerve) and intramuscular saline (0.2mL/kg) (GCON); perineural administration of dexmedetomidine diluted (1µg/mL) in ropivacaine 0.75% on the aforementioned nerves (0.1mL/kg/nerve) and intramuscular saline (0.2mL/kg) (GDPN); perineural administration of ropivacaine 0.75% on the aforementioned nerves (0.1mL/kg/nerve) and intramuscular dexmedetomidine diluted (1µg/mL) in saline (0.2mL/kg) (GDIM). The blocks will be performed under general inhalation anesthesia and guided by ultrasound and electrical nerve stimulation. Afterward, the dogs will be allowed to recover from general anesthesia. The variables Heart Rate (HR), Respiratory Rate (RR), Systolic Arterial Pressure (SAP), Rectal Temperature (RT), Motor (Amot) and Sensory (Asen) Activity will be evaluated before Anesthetic Induction (MB), immediately following Anesthetic Recovery (M0) and every 15 minutes (M15, M30, M45&) until total recovery of motor and sensory activity. The variables arterial partial pressure of oxygen (PaO2), Arterial Partial Pressure of Carbon Dioxide (PaCO2), blood pH, Arterial Oxygen Saturation (SaO2), bicarbonate (HCO3-), base deficit (BE), Blood Lactate (LACT) and Blood Glucose (GLIC) will be evaluated at MB, M0, 180 and 540 minutes following anesthetic recovery. Twenty-four and 72 hours following M0, the variables Amot and Asen will be evaluated again to assess for potential neurological deficits in the treated limb. Data will be tested for normality using Shapiro-Wilk. The variables presenting normal distribution will submitted to repeated measures Analysis of Variance (ANOVA) and Tukey's post hoc test. The variables without normal distribution will be analyzed with Friedman's test and Student-Newman-Keuls' post hoc test. Differences between treatments will be considered statistically significant when p<0.05. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TREIN, THOMAS A.; FLORIANO, BEATRIZ P.; WAGATSUMA, JULIANA T.; FERREIRA, JOANA Z.; DA SILVA, GUILHERME L.; DOS SANTOS, PAULO S. P.; PERRI, SILVIA H. V.; OLIVA, VALRIA N. L. S. Effects of dexmedetomidine combined with ropivacaine on sciatic and femoral nerve blockade in dogs. VETERINARY ANAESTHESIA AND ANALGESIA, v. 44, n. 1, p. 144-153, JAN 2017. Web of Science Citations: 4.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TREIN, Thomas Alexander. Administração da associação de dexmedetomidina e ropivacaína 0,75% em bloqueios dos nervos isquiático e femoral guiados por ultrassom e por neuroeletroestimulação em cães. 2015. 162 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Medicina Veterinária..

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